Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4011647..4012431 Replicon chromosome
Accession NZ_CP053851
Organism Escherichia coli strain CE1803A

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag HPM97_RS18780 Protein ID WP_000613626.1
Coordinates 4011647..4012141 (-) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag HPM97_RS18785 Protein ID WP_001110447.1
Coordinates 4012138..4012431 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPM97_RS18760 4006840..4007937 + 1098 WP_000589326.1 flagellar basal body P-ring protein FlgI -
HPM97_RS18765 4007937..4008878 + 942 WP_001305885.1 flagellar assembly peptidoglycan hydrolase FlgJ -
HPM97_RS18770 4008944..4010587 + 1644 WP_061092652.1 flagellar hook-associated protein FlgK -
HPM97_RS18775 4010599..4011552 + 954 WP_001212762.1 flagellar hook-associated protein FlgL -
HPM97_RS18780 4011647..4012141 - 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
HPM97_RS18785 4012138..4012431 - 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
HPM97_RS18790 4012564..4015749 - 3186 WP_061092653.1 ribonuclease E -
HPM97_RS18795 4016322..4017281 + 960 WP_000846342.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T158121 WP_000613626.1 NZ_CP053851:c4012141-4011647 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

>T158121 NZ_CP069583:85848-85955 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT158121 WP_001110447.1 NZ_CP053851:c4012431-4012138 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp

>AT158121 NZ_CP069583:c85799-85734 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References