Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2949564..2950218 Replicon chromosome
Accession NZ_CP053851
Organism Escherichia coli strain CE1803A

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag HPM97_RS13980 Protein ID WP_000244781.1
Coordinates 2949811..2950218 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HPM97_RS13975 Protein ID WP_000354046.1
Coordinates 2949564..2949830 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPM97_RS13955 2945652..2947085 - 1434 WP_001361333.1 6-phospho-beta-glucosidase BglA -
HPM97_RS13960 2947130..2947441 + 312 WP_001182965.1 N(4)-acetylcytidine aminohydrolase -
HPM97_RS13965 2947605..2948264 + 660 WP_000250274.1 hemolysin III family protein -
HPM97_RS13970 2948341..2949321 - 981 WP_000886072.1 tRNA-modifying protein YgfZ -
HPM97_RS13975 2949564..2949830 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HPM97_RS13980 2949811..2950218 + 408 WP_000244781.1 protein YgfX Toxin
HPM97_RS13985 2950258..2950779 - 522 WP_001055874.1 flavodoxin FldB -
HPM97_RS13990 2950891..2951787 + 897 WP_000806650.1 site-specific tyrosine recombinase XerD -
HPM97_RS13995 2951812..2952522 + 711 WP_000715220.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HPM97_RS14000 2952528..2954261 + 1734 WP_000813184.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T158119 WP_000244781.1 NZ_CP053851:2949811-2950218 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T158119 NZ_CP069582:2165844-2166050 [Staphylococcus carnosus]
ATGCAAAATAGTGTAATTAAAATTGATGGAATGGAAACTGAAGAAGAACAGCATAAATTGCATCAACATCTTATAGAAAT
GACAGGTGTATCTGCAGTGCAAGTGGATATGGATTCAAATGAAGTCAGAATCAGTTATGAAACACCTGTAAATCTCAATA
ATCTTGAAAAAGAAATCTATGATGCAGGTTATCAAATATTAAATTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT158119 WP_000354046.1 NZ_CP053851:2949564-2949830 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT158119 NZ_CP069582:2165539-2165832 [Staphylococcus carnosus]
ATGACCGAAAATGATAAAGCGCACCATTCAGAACAAATCAAATCCAACTTGAAAGCACGACTCAATCGAATTGAAGGACA
AGTCAGAGCAATTAACCGAATGATTGAAGAGGATGTATATTGTGATGATGTACTCACACAAATCCGTGCAACACGTTCAG
CTTTGAACAGTGTGGCAGTACGATTGCTTGATCATCACATGAAAGGTTGTATCATGCATAAAGTTGAGGATGGTCACGCT
GAAGAAGCGATGGAAGAATTACTTGTTACCGTTCAAAAATTGATTAAAGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References