Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2305985..2306616 Replicon chromosome
Accession NZ_CP053851
Organism Escherichia coli strain CE1803A

Toxin (Protein)


Gene name hicA Uniprot ID L4J012
Locus tag HPM97_RS10770 Protein ID WP_001260301.1
Coordinates 2305985..2306260 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag HPM97_RS10775 Protein ID WP_000593555.1
Coordinates 2306257..2306616 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPM97_RS10755 2300989..2302056 + 1068 WP_000361497.1 HlyD family efflux transporter periplasmic adaptor subunit -
HPM97_RS10760 2302053..2304788 + 2736 WP_061092822.1 ribosome-associated ATPase/putative transporter RbbA -
HPM97_RS10765 2304788..2305912 + 1125 WP_001314210.1 ABC transporter permease -
HPM97_RS10770 2305985..2306260 + 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin Toxin
HPM97_RS10775 2306257..2306616 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HPM97_RS10780 2306736..2307137 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
HPM97_RS10785 2307142..2307948 - 807 WP_061092823.1 nickel import ATP-binding protein NikE -
HPM97_RS10790 2307945..2308709 - 765 WP_001136211.1 nickel import ATP-binding protein NikD -
HPM97_RS10795 2308709..2309542 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
HPM97_RS10800 2309539..2310483 - 945 WP_000947080.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2299001..2307137 8136


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10185.87 Da        Isoelectric Point: 10.6128

>T158116 WP_001260301.1 NZ_CP053851:2305985-2306260 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T158116 NZ_CP069581:c18213-18118 [Staphylococcus carnosus]
ATGTTGGAAATCCTTGTTCACATCACGACCACAGTCATCAGTGGTTGTATTATTGCGTTATTTACGCATTGGCTGCGTAA
TCGCAAAGACAAATAG

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT158116 WP_000593555.1 NZ_CP053851:2306257-2306616 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT158116 NZ_CP069581:c18100-18065 [Staphylococcus carnosus]
TACAAAAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB L4J012

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References