Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1778294..1778910 Replicon chromosome
Accession NZ_CP053851
Organism Escherichia coli strain CE1803A

Toxin (Protein)


Gene name vapC Uniprot ID A0A176ZIX8
Locus tag HPM97_RS08430 Protein ID WP_001129490.1
Coordinates 1778294..1778668 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1M0CY48
Locus tag HPM97_RS08435 Protein ID WP_001275523.1
Coordinates 1778668..1778910 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPM97_RS08415 1775797..1776699 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
HPM97_RS08420 1776696..1777331 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
HPM97_RS08425 1777328..1778257 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
HPM97_RS08430 1778294..1778668 - 375 WP_001129490.1 type II toxin-antitoxin system VapC family toxin Toxin
HPM97_RS08435 1778668..1778910 - 243 WP_001275523.1 ribbon-helix-helix domain-containing protein Antitoxin
HPM97_RS08440 1779130..1779348 - 219 WP_001251290.1 ribbon-helix-helix domain-containing protein -
HPM97_RS08445 1780022..1781836 - 1815 WP_000226324.1 hypothetical protein -
HPM97_RS08450 1782252..1783193 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
HPM97_RS08455 1783238..1783675 - 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-115)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-48)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13960.19 Da        Isoelectric Point: 7.0126

>T158114 WP_001129490.1 NZ_CP053851:c1778668-1778294 [Escherichia coli]
MAKRTALFDTNILIDLFSGRIEAKQALEAWPLQNAISLVTWMEVLVGAKKYDQEHRTRIAMSAFNVIGISQDIAERSVAL
RQEYRMKLPDAIILATAQIYGFELVTRNTRDFAGIAGVVTPYEL

Download         Length: 375 bp

>T158114 NZ_CP069572:c68870-68712 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9290.54 Da        Isoelectric Point: 4.3774

>AT158114 WP_001275523.1 NZ_CP053851:c1778910-1778668 [Escherichia coli]
MSAMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVDQYLENQSKTTISSAFGIWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT158114 NZ_CP069572:c69138-68914 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A176ZIX8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M0CY48

References