158109

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 715934..716771 Replicon chromosome
Accession NZ_CP053851
Organism Escherichia coli strain CE1803A

Toxin (Protein)


Gene name itaT Uniprot ID A0A1M0D5U3
Locus tag HPM97_RS03370 Protein ID WP_000227782.1
Coordinates 716229..716771 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID B1LJI1
Locus tag HPM97_RS03365 Protein ID WP_001353405.1
Coordinates 715934..716245 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPM97_RS03340 710954..711901 + 948 WP_001239437.1 cytochrome o ubiquinol oxidase subunit II -
HPM97_RS03345 711923..713914 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
HPM97_RS03350 713904..714518 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
HPM97_RS03355 714518..714847 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
HPM97_RS03360 714859..715749 + 891 WP_000971327.1 heme o synthase -
HPM97_RS03365 715934..716245 + 312 WP_001353405.1 DUF1778 domain-containing protein Antitoxin
HPM97_RS03370 716229..716771 + 543 WP_000227782.1 GNAT family N-acetyltransferase Toxin
HPM97_RS03375 716827..717671 - 845 Protein_655 sel1 repeat family protein -
HPM97_RS03380 718078..719442 + 1365 WP_001000978.1 MFS transporter -
HPM97_RS03385 719570..720061 - 492 WP_001138904.1 nucleotide binding protein YajQ -
HPM97_RS03390 720229..721140 + 912 WP_000705841.1 2-dehydropantoate 2-reductase -
HPM97_RS03395 721103..721693 + 591 WP_001276320.1 protein deglycase YajL -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(19-96)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19957.31 Da        Isoelectric Point: 9.1763

>T158109 WP_000227782.1 NZ_CP053851:716229-716771 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDIIYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCNFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T158109 NZ_CP069571:4561969-4562187 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11370.09 Da        Isoelectric Point: 10.4825

>AT158109 WP_001353405.1 NZ_CP053851:715934-716245 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT158109 NZ_CP069571:4561569-4561943 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M0D5U3

Antitoxin

Source ID Structure
AlphaFold DB A0A829G7G3

References