Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 681844..682462 Replicon chromosome
Accession NZ_CP053851
Organism Escherichia coli strain CE1803A

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HPM97_RS03200 Protein ID WP_001291435.1
Coordinates 682244..682462 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HPM97_RS03195 Protein ID WP_000344800.1
Coordinates 681844..682218 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPM97_RS03185 676933..678126 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HPM97_RS03190 678149..681298 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
HPM97_RS03195 681844..682218 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HPM97_RS03200 682244..682462 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
HPM97_RS03205 682634..683185 + 552 WP_000102574.1 maltose O-acetyltransferase -
HPM97_RS03210 683301..683771 + 471 WP_000136192.1 YlaC family protein -
HPM97_RS03215 683935..685485 + 1551 WP_001361689.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HPM97_RS03220 685527..685880 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HPM97_RS03230 686259..686570 + 312 WP_000409911.1 MGMT family protein -
HPM97_RS03235 686601..687173 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T158108 WP_001291435.1 NZ_CP053851:682244-682462 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T158108 NZ_CP069571:3757688-3757795 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT158108 WP_000344800.1 NZ_CP053851:681844-682218 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT158108 NZ_CP069571:c3757641-3757575 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References