Detailed information of TA system TA158100

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1001946..1002520
• Replicon: chromosome
• Accession: NZ_CP053833
• Organism: Arcobacter cloacae strain LMG 26153

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: ACLO_RS05095
• Protein ID: WP_129012588.1
• Coordinates: 1001946..1002281 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: ACLO_RS05100
• Protein ID: WP_129012589.1
• Coordinates: 1002275..1002520 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACLO_RS05070	997079..997495	-	417	WP_129012585.1	DUF721 domain-containing protein	-
ACLO_RS05075	997466..997612	+	147	WP_164967033.1	hypothetical protein	-
ACLO_RS05080	997609..999063	+	1455	WP_128985552.1	FAD-binding protein	-
ACLO_RS05085	999187..1000722	+	1536	WP_129012586.1	glutamine-hydrolyzing GMP synthase	-
ACLO_RS05090	1000930..1001892	+	963	WP_129012587.1	hypothetical protein	-
ACLO_RS05095	1001946..1002281	-	336	WP_129012588.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ACLO_RS05100	1002275..1002520	-	246	WP_129012589.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ACLO_RS05105	1002651..1003112	+	462	WP_129012590.1	hypothetical protein	-
ACLO_RS05110	1003305..1005002	-	1698	WP_129012591.1	YdiU family protein	-
ACLO_RS05115	1005002..1005805	-	804	WP_129012592.1	DnaJ domain-containing protein	-
ACLO_RS05120	1005974..1007338	+	1365	WP_129012593.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	989628..1268161	278533

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-103)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12531.69 Da
• Isoelectric Point: 10.1692

>T158100 WP_129012588.1 NZ_CP053833:c1002281-1001946 [Arcobacter cloacae]
MVKNYIPKKGDLVILSFDPSSGHEQKGRRPALIISNEIFNKALGLAIACPITNTDRNFPFHVKLEAKNLKGFIMTEQIKS
IDFNARKVKFVEKIDEDTLNQVLGITKSIIF

Nucleotide

• Length: 336 bp

>T158100 NZ_CP069571:1829807-1830103 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9211.53 Da
• Isoelectric Point: 4.4678

>AT158100 WP_129012589.1 NZ_CP053833:c1002520-1002275 [Arcobacter cloacae]
MTAKISKWGNSQGLRMPKDVMESLHLNVGDDVNIIVFDGKVILEPIKKEILNYDLNELVSKIPSDYKASEEFDTTVGKEE
W

Nucleotide

• Length: 246 bp

>AT158100 NZ_CP069571:1830105-1830500 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA