Detailed information of TA system TA158093

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-Phd
• Location: 495122..495700
• Replicon: chromosome
• Accession: NZ_CP053823
• Organism: Vibrio cholerae strain E7G

Toxin (Protein)

• Gene name: parE
• Uniprot ID: O68848
• Locus tag: HPY10_RS16420
• Protein ID: WP_001180243.1
• Coordinates: 495122..495439 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: HPY10_RS16425
• Protein ID: WP_001961623.1
• Coordinates: 495458..495700 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY10_RS16390	490216..490353	+	138	WP_175246445.1	DUF3265 domain-containing protein	-
HPY10_RS16395	490390..491091	+	702	WP_001175917.1	hypothetical protein	-
HPY10_RS16400	491329..492732	+	1404	WP_175246446.1	AAA family ATPase	-
HPY10_RS16405	492725..493276	+	552	WP_175246447.1	hypothetical protein	-
HPY10_RS16410	493589..493770	+	182	Protein_573	DUF645 family protein	-
HPY10_RS16415	493983..494987	+	1005	WP_000964932.1	LD-carboxypeptidase	-
HPY10_RS16420	495122..495439	-	318	WP_001180243.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HPY10_RS16425	495458..495700	-	243	WP_001961623.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HPY10_RS16430	496162..496272	+	111	WP_076025930.1	DUF645 family protein	-
HPY10_RS16435	496423..497157	+	735	WP_114716399.1	hypothetical protein	-
HPY10_RS16440	497321..498061	+	741	WP_162813548.1	PhzF family phenazine biosynthesis protein	-
HPY10_RS16445	498202..498777	+	576	WP_000199096.1	class I SAM-dependent methyltransferase	-
HPY10_RS16450	498952..499284	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HPY10_RS16455	499271..499585	+	315	WP_000071008.1	DNA-binding transcriptional regulator	-
HPY10_RS16460	499582..499692	+	111	WP_080342631.1	DUF3265 domain-containing protein	-
HPY10_RS16465	499700..500017	-	318	WP_000077453.1	CcdB family protein	-
HPY10_RS16470	500017..500262	-	246	WP_001260801.1	type II toxin-antitoxin system CcdA family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	310054..527444	217390
	-	inside	Integron	-	-	311218..527444	216226

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-93)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-71)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12158.97 Da
• Isoelectric Point: 9.7495

>T158093 WP_001180243.1 NZ_CP053823:c495439-495122 [Vibrio cholerae]
MQNKQYKLSQLAQEHLLKIKHYTIENFAEAQWQKYKSTLLSGFQTLADNPGLGKSCEDIYQNGFYFPVGKHMAYYTKEAN
FILIVAVLGQSQLPQKHLKQSRFVS

Nucleotide

• Length: 318 bp

>T158093 NZ_CP069571:c338196-337909 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGTCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8931.22 Da
• Isoelectric Point: 5.6630

>AT158093 WP_001961623.1 NZ_CP053823:c495700-495458 [Vibrio cholerae]
MHTLTANDAKRNFGELLLSAQREPVIISKNSKNTVVVMSIKDFEELEAMKLDYLKHCFESAQKDLDGGKTVDGATFLNTL

Nucleotide

• Length: 243 bp

>AT158093 NZ_CP069571:c337922-337620 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A0K9UJR8