Detailed information of TA system TA158092

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 488711..489209
• Replicon: chromosome
• Accession: NZ_CP053823
• Organism: Vibrio cholerae strain E7G

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: HPY10_RS16370
• Protein ID: WP_000589156.1
• Coordinates: 488711..488977 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: HPY10_RS16375
• Protein ID: WP_000643598.1
• Coordinates: 488964..489209 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY10_RS16325	484176..484601	+	426	WP_000415750.1	hypothetical protein	-
HPY10_RS16330	484949..485130	+	182	Protein_557	DUF645 family protein	-
HPY10_RS16335	485622..485828	+	207	WP_175247674.1	DUF3265 domain-containing protein	-
HPY10_RS16340	485831..486151	+	321	WP_175247675.1	DUF1778 domain-containing protein	-
HPY10_RS16345	486148..486681	+	534	WP_175246444.1	GNAT family N-acetyltransferase	-
HPY10_RS16350	486987..487169	+	183	WP_001933694.1	DUF645 family protein	-
HPY10_RS16355	487318..487923	+	606	WP_175242423.1	suppressor of fused domain protein	-
HPY10_RS16360	487928..488038	+	111	WP_080100071.1	DUF3265 domain-containing protein	-
HPY10_RS16365	488079..488534	+	456	WP_000432865.1	hypothetical protein	-
HPY10_RS16370	488711..488977	+	267	WP_000589156.1	BrnT family toxin	Toxin
HPY10_RS16375	488964..489209	+	246	WP_000643598.1	hypothetical protein	Antitoxin
HPY10_RS16380	489684..489794	+	111	WP_086017781.1	DUF3265 domain-containing protein	-
HPY10_RS16385	489836..490246	+	411	WP_025613414.1	cytidine deaminase	-
HPY10_RS16390	490216..490353	+	138	WP_175246445.1	DUF3265 domain-containing protein	-
HPY10_RS16395	490390..491091	+	702	WP_001175917.1	hypothetical protein	-
HPY10_RS16400	491329..492732	+	1404	WP_175246446.1	AAA family ATPase	-
HPY10_RS16405	492725..493276	+	552	WP_175246447.1	hypothetical protein	-
HPY10_RS16410	493589..493770	+	182	Protein_573	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	310054..527444	217390
	-	inside	Integron	-	-	311218..527444	216226

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T158092 WP_000589156.1 NZ_CP053823:488711-488977 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T158092 NZ_CP069571:27359-27637 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAA
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT158092 WP_000643598.1 NZ_CP053823:488964-489209 [Vibrio cholerae]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT158092 NZ_CP069571:27096-27356 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: A0A271VME2