Detailed information of TA system TA158091

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 485831..486681
• Replicon: chromosome
• Accession: NZ_CP053823
• Organism: Vibrio cholerae strain E7G

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HPY10_RS16345
• Protein ID: WP_175246444.1
• Coordinates: 486148..486681 (+)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HPY10_RS16340
• Protein ID: WP_175247675.1
• Coordinates: 485831..486151 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY10_RS16305	481270..481647	+	378	WP_175246443.1	hypothetical protein	-
HPY10_RS16310	481805..482230	+	426	WP_001887505.1	GNAT family N-acetyltransferase	-
HPY10_RS16315	482287..483141	+	855	WP_116672171.1	aminoglycoside phosphotransferase family protein	-
HPY10_RS16320	483301..483759	+	459	WP_114755989.1	hypothetical protein	-
HPY10_RS16325	484176..484601	+	426	WP_000415750.1	hypothetical protein	-
HPY10_RS16330	484949..485130	+	182	Protein_557	DUF645 family protein	-
HPY10_RS16335	485622..485828	+	207	WP_175247674.1	DUF3265 domain-containing protein	-
HPY10_RS16340	485831..486151	+	321	WP_175247675.1	DUF1778 domain-containing protein	Antitoxin
HPY10_RS16345	486148..486681	+	534	WP_175246444.1	GNAT family N-acetyltransferase	Toxin
HPY10_RS16350	486987..487169	+	183	WP_001933694.1	DUF645 family protein	-
HPY10_RS16355	487318..487923	+	606	WP_175242423.1	suppressor of fused domain protein	-
HPY10_RS16360	487928..488038	+	111	WP_080100071.1	DUF3265 domain-containing protein	-
HPY10_RS16365	488079..488534	+	456	WP_000432865.1	hypothetical protein	-
HPY10_RS16370	488711..488977	+	267	WP_000589156.1	BrnT family toxin	-
HPY10_RS16375	488964..489209	+	246	WP_000643598.1	hypothetical protein	-
HPY10_RS16380	489684..489794	+	111	WP_086017781.1	DUF3265 domain-containing protein	-
HPY10_RS16385	489836..490246	+	411	WP_025613414.1	cytidine deaminase	-
HPY10_RS16390	490216..490353	+	138	WP_175246445.1	DUF3265 domain-containing protein	-
HPY10_RS16395	490390..491091	+	702	WP_001175917.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	310054..527444	217390
	-	inside	Integron	-	-	311218..527444	216226

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (22-98)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 20196.56 Da
• Isoelectric Point: 9.6050

>T158091 WP_175246444.1 NZ_CP053823:486148-486681 [Vibrio cholerae]
VSWGKEFVELSKSKHDRDSFDCGEQELNTFIKTQAAKHMQAGISRTMVLPSAHPLLNQKFAICAFYSVAPSSISRETLPA
QLAKKLPRYPIPVFLLAQLAVHKKFHGSGLGKVSLIRALKYLWEVNYHMRAYAIVVDCLTDSAQAFYTKFGFEVLCEHNE
RIRMFLPMKTVEQLFNQ

Nucleotide

• Length: 534 bp

>T158091 NZ_CP069571:c21305-20907 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 11717.45 Da
• Isoelectric Point: 4.9309

>AT158091 WP_175247675.1 NZ_CP053823:485831-486151 [Vibrio cholerae]
MAYNRSTDGMYPYAGGGMATARLDIRLDEEIKAKAEKASALLGLKSLTEYVVRLMDEDATHVIEEHESIMVKDSVFDEFM
AACNKVKAPNQALLEAVKFTEKSGIK

Nucleotide

• Length: 321 bp

>AT158091 NZ_CP069571:c21601-21308 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA