Detailed information of TA system TA158078

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 341157..341655
• Replicon: chromosome
• Accession: NZ_CP053823
• Organism: Vibrio cholerae strain E7G

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: HPY10_RS15075
• Protein ID: WP_000589156.1
• Coordinates: 341157..341423 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: HPY10_RS15080
• Protein ID: WP_000643598.1
• Coordinates: 341410..341655 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY10_RS15035	336621..336740	+	120	WP_096037231.1	DUF3265 domain-containing protein	-
HPY10_RS15040	337180..337305	+	126	WP_175247722.1	DUF3265 domain-containing protein	-
HPY10_RS15045	337333..337812	+	480	WP_175247632.1	peptidase T	-
HPY10_RS15050	337981..339447	+	1467	WP_175247633.1	hypothetical protein	-
HPY10_RS15055	339808..339918	+	111	Protein_302	DUF645 family protein	-
HPY10_RS15060	340153..340575	+	423	WP_130243470.1	hypothetical protein	-
HPY10_RS15065	340719..340946	+	228	WP_045612249.1	hypothetical protein	-
HPY10_RS15070	340961..341101	+	141	Protein_305	DUF3265 domain-containing protein	-
HPY10_RS15075	341157..341423	+	267	WP_000589156.1	BrnT family toxin	Toxin
HPY10_RS15080	341410..341655	+	246	WP_000643598.1	hypothetical protein	Antitoxin
HPY10_RS15085	341833..342117	+	285	WP_000048765.1	antibiotic biosynthesis monooxygenase	-
HPY10_RS15090	342320..342709	+	390	WP_000491261.1	VOC family protein	-
HPY10_RS15095	342860..343294	+	435	WP_175247634.1	GNAT family N-acetyltransferase	-
HPY10_RS15100	343454..343735	+	282	WP_000382665.1	hypothetical protein	-
HPY10_RS15105	343808..344272	-	465	WP_055065351.1	hypothetical protein	-
HPY10_RS15110	344610..344927	+	318	WP_000640723.1	heavy metal-binding domain-containing protein	-
HPY10_RS15115	344924..345034	+	111	WP_071936816.1	DUF3265 domain-containing protein	-
HPY10_RS15120	345107..345430	+	324	WP_080476600.1	DUF3709 domain-containing protein	-
HPY10_RS15125	345697..346233	+	537	WP_019829641.1	nucleotidyltransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	310054..527444	217390
	-	inside	Integron	-	-	311218..527444	216226

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T158078 WP_000589156.1 NZ_CP053823:341157-341423 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T158078 NZ_CP069565:945143-945409 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT158078 WP_000643598.1 NZ_CP053823:341410-341655 [Vibrio cholerae]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT158078 NZ_CP069565:944892-945143 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGTTATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: A0A271VME2