Detailed information of TA system TA158065

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 448550..449048
• Replicon: chromosome
• Accession: NZ_CP053821
• Organism: Vibrio cholerae strain SA10G

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: HPY11_RS15995
• Protein ID: WP_000589156.1
• Coordinates: 448550..448816 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: HPY11_RS16000
• Protein ID: WP_000643598.1
• Coordinates: 448803..449048 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY11_RS15950	443611..444069	+	459	WP_114755989.1	hypothetical protein	-
HPY11_RS15955	444486..444911	+	426	WP_000415750.1	hypothetical protein	-
HPY11_RS15960	445259..445440	+	182	Protein_474	DUF645 family protein	-
HPY11_RS15965	445589..445990	+	402	WP_175246608.1	DUF1778 domain-containing protein	-
HPY11_RS15970	445987..446520	+	534	WP_175246444.1	GNAT family N-acetyltransferase	-
HPY11_RS15975	446826..447008	+	183	WP_001933694.1	DUF645 family protein	-
HPY11_RS15980	447157..447762	+	606	WP_175242423.1	suppressor of fused domain protein	-
HPY11_RS15985	447767..447877	+	111	WP_080100071.1	DUF3265 domain-containing protein	-
HPY11_RS15990	447918..448373	+	456	WP_000432865.1	hypothetical protein	-
HPY11_RS15995	448550..448816	+	267	WP_000589156.1	BrnT family toxin	Toxin
HPY11_RS16000	448803..449048	+	246	WP_000643598.1	hypothetical protein	Antitoxin
HPY11_RS16005	449523..449633	+	111	WP_086017781.1	DUF3265 domain-containing protein	-
HPY11_RS16010	449675..450085	+	411	WP_025613414.1	cytidine deaminase	-
HPY11_RS16015	450055..450192	+	138	WP_175246445.1	DUF3265 domain-containing protein	-
HPY11_RS16020	450229..450930	+	702	WP_001175917.1	hypothetical protein	-
HPY11_RS16025	451168..452571	+	1404	WP_175246446.1	AAA family ATPase	-
HPY11_RS16030	452564..453115	+	552	WP_175246447.1	hypothetical protein	-
HPY11_RS16035	453428..453609	+	182	Protein_489	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	310496..487283	176787
	-	inside	Integron	-	-	311660..487283	175623

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T158065 WP_000589156.1 NZ_CP053821:448550-448816 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T158065 NZ_CP069559:c4528452-4528243 [Escherichia coli]
ATGGTTGATTATCCAAAACGTGATGCTATTTCCAACATCAACGCTATAATTGTAGATACATTTTTGTATATACAATTAGG
GATGGCAACTATGCCAATGGAGTTTGAATGGAATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTG
AAGATGCGGTGTTAGTGCTTGATGATCCACAACGAACGGAGCCGCTATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT158065 WP_000643598.1 NZ_CP053821:448803-449048 [Vibrio cholerae]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT158065 NZ_CP069559:c4528246-4527944 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGTGCTGGCGAA
CAAATTCGACGAGGATATTGATTACAGCGACATCCCATCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: A0A271VME2