Detailed information of TA system TA158064

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 445589..446520
• Replicon: chromosome
• Accession: NZ_CP053821
• Organism: Vibrio cholerae strain SA10G

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HPY11_RS15970
• Protein ID: WP_175246444.1
• Coordinates: 445987..446520 (+)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HPY11_RS15965
• Protein ID: WP_175246608.1
• Coordinates: 445589..445990 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY11_RS15930	440954..441430	+	477	WP_000776932.1	DM13 domain-containing protein	-
HPY11_RS15935	441580..441957	+	378	WP_175246443.1	hypothetical protein	-
HPY11_RS15940	442115..442540	+	426	WP_001887505.1	GNAT family N-acetyltransferase	-
HPY11_RS15945	442597..443451	+	855	WP_116672171.1	aminoglycoside phosphotransferase family protein	-
HPY11_RS15950	443611..444069	+	459	WP_114755989.1	hypothetical protein	-
HPY11_RS15955	444486..444911	+	426	WP_000415750.1	hypothetical protein	-
HPY11_RS15960	445259..445440	+	182	Protein_474	DUF645 family protein	-
HPY11_RS15965	445589..445990	+	402	WP_175246608.1	DUF1778 domain-containing protein	Antitoxin
HPY11_RS15970	445987..446520	+	534	WP_175246444.1	GNAT family N-acetyltransferase	Toxin
HPY11_RS15975	446826..447008	+	183	WP_001933694.1	DUF645 family protein	-
HPY11_RS15980	447157..447762	+	606	WP_175242423.1	suppressor of fused domain protein	-
HPY11_RS15985	447767..447877	+	111	WP_080100071.1	DUF3265 domain-containing protein	-
HPY11_RS15990	447918..448373	+	456	WP_000432865.1	hypothetical protein	-
HPY11_RS15995	448550..448816	+	267	WP_000589156.1	BrnT family toxin	-
HPY11_RS16000	448803..449048	+	246	WP_000643598.1	hypothetical protein	-
HPY11_RS16005	449523..449633	+	111	WP_086017781.1	DUF3265 domain-containing protein	-
HPY11_RS16010	449675..450085	+	411	WP_025613414.1	cytidine deaminase	-
HPY11_RS16015	450055..450192	+	138	WP_175246445.1	DUF3265 domain-containing protein	-
HPY11_RS16020	450229..450930	+	702	WP_001175917.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	310496..487283	176787
	-	inside	Integron	-	-	311660..487283	175623

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (49-125)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 20196.56 Da
• Isoelectric Point: 9.6050

>T158064 WP_175246444.1 NZ_CP053821:445987-446520 [Vibrio cholerae]
VSWGKEFVELSKSKHDRDSFDCGEQELNTFIKTQAAKHMQAGISRTMVLPSAHPLLNQKFAICAFYSVAPSSISRETLPA
QLAKKLPRYPIPVFLLAQLAVHKKFHGSGLGKVSLIRALKYLWEVNYHMRAYAIVVDCLTDSAQAFYTKFGFEVLCEHNE
RIRMFLPMKTVEQLFNQ

Nucleotide

• Length: 534 bp

>T158064 NZ_CP069559:4459437-4459589 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14813.17 Da
• Isoelectric Point: 7.7413

>AT158064 WP_175246608.1 NZ_CP053821:445589-445990 [Vibrio cholerae]
MRCQPLRRALYSPSPRKLSVRIECALSLAYNRSTDGMYPYAGGGMATARLDIRLDEEIKAKAEKASALLGLKSLTEYVVR
LMDEDATHVIEEHESIMVKDSVFDEFMAACNKVKAPNQALLEAVKFTEKSGIK

Nucleotide

• Length: 402 bp

>AT158064 NZ_CP069559:c4459388-4459331 [Escherichia coli]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA