Detailed information of TA system TA158055

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 352087..352585
• Replicon: chromosome
• Accession: NZ_CP053821
• Organism: Vibrio cholerae strain SA10G

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: HPY11_RS15165
• Protein ID: WP_000589156.1
• Coordinates: 352087..352353 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: D7HCY3
• Locus tag: HPY11_RS15170
• Protein ID: WP_000643600.1
• Coordinates: 352340..352585 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY11_RS15120	347126..347413	+	288	WP_000869999.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HPY11_RS15125	347545..347964	+	420	WP_058666901.1	hypothetical protein	-
HPY11_RS15130	347961..348071	+	111	Protein_308	DUF3265 domain-containing protein	-
HPY11_RS15135	348564..349280	+	717	WP_071186049.1	GIY-YIG nuclease family protein	-
HPY11_RS15140	349416..349952	+	537	WP_005526589.1	nucleotidyltransferase family protein	-
HPY11_RS15145	350121..350384	+	264	WP_053543595.1	hypothetical protein	-
HPY11_RS15150	350667..350837	+	171	WP_175246407.1	DUF645 family protein	-
HPY11_RS15155	351248..351343	+	96	WP_001907607.1	DUF645 family protein	-
HPY11_RS15160	351698..351851	+	154	Protein_314	DUF645 family protein	-
HPY11_RS15165	352087..352353	+	267	WP_000589156.1	BrnT family toxin	Toxin
HPY11_RS15170	352340..352585	+	246	WP_000643600.1	hypothetical protein	Antitoxin
HPY11_RS15175	353247..353606	+	360	WP_142728166.1	HNH endonuclease	-
HPY11_RS15180	353781..354248	+	468	WP_101412260.1	DUF1643 domain-containing protein	-
HPY11_RS15185	354410..355909	-	1500	WP_175246408.1	hypothetical protein	-
HPY11_RS15190	356225..356377	+	153	WP_114774755.1	DUF645 family protein	-
HPY11_RS15195	356732..356818	+	87	WP_172778914.1	DUF645 family protein	-
HPY11_RS15200	356812..356910	+	99	WP_134818269.1	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	310496..487283	176787
	-	inside	Integron	-	-	311660..487283	175623

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T158055 WP_000589156.1 NZ_CP053821:352087-352353 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T158055 NZ_CP069559:2283367-2283618 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9446.71 Da
• Isoelectric Point: 6.4832

>AT158055 WP_000643600.1 NZ_CP053821:352340-352585 [Vibrio cholerae]
MKAHEFDAKFESDDDDIVVDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT158055 NZ_CP069559:2283615-2283869 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGAAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGATCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: D7HCY3