Detailed information of TA system TA158051

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 23275..23932
• Replicon: plasmid pSA7G1
• Accession: NZ_CP053818
• Organism: Vibrio cholerae strain SA7G

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HPY17_RS19475
• Protein ID: WP_175247884.1
• Coordinates: 23275..23682 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: HPY17_RS19480
• Protein ID: WP_175247819.1
• Coordinates: 23696..23932 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY17_RS19435	18496..19140	-	645	WP_175247812.1	hypothetical protein	-
HPY17_RS19440	19137..19487	-	351	WP_175247813.1	hypothetical protein	-
HPY17_RS19445	19514..19951	-	438	WP_175247814.1	hypothetical protein	-
HPY17_RS19450	20151..20294	-	144	WP_175247815.1	hypothetical protein	-
HPY17_RS19455	20326..20646	-	321	WP_175247816.1	hypothetical protein	-
HPY17_RS19460	20988..21773	-	786	WP_088412287.1	site-specific integrase	-
HPY17_RS19465	22277..22588	-	312	WP_175247817.1	hypothetical protein	-
HPY17_RS19470	22739..23278	-	540	WP_175247818.1	hypothetical protein	-
HPY17_RS19475	23275..23682	-	408	WP_175247884.1	type II toxin-antitoxin system VapC family toxin	Toxin
HPY17_RS19480	23696..23932	-	237	WP_175247819.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HPY17_RS19485	24207..25403	-	1197	WP_175247820.1	replication initiation protein	-
HPY17_RS19490	25527..25823	-	297	WP_175247821.1	hypothetical protein	-
HPY17_RS19495	26234..26572	-	339	WP_175247822.1	hypothetical protein	-
HPY17_RS19500	26617..26931	-	315	WP_088412263.1	hypothetical protein	-
HPY17_RS19505	26935..27291	-	357	WP_175247823.1	hypothetical protein	-
HPY17_RS19510	27266..27508	-	243	WP_175247824.1	hypothetical protein	-
HPY17_RS19515	27603..27854	-	252	WP_175247825.1	hypothetical protein	-
HPY17_RS19520	28004..28321	+	318	WP_114729331.1	hypothetical protein	-
HPY17_RS19525	28472..28714	+	243	WP_175247826.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..79397	79397

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• SpoVT-AbrB domain (8-51)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14998.41 Da
• Isoelectric Point: 8.2818

>T158051 WP_175247884.1 NZ_CP053818:c23682-23275 [Vibrio cholerae]
MLDTCICSFIMREQPTSVLEKLQEIVSKRHRVVISVVTYQEMQYGLLGKKAAPKHAVLVEEFLKRVDAILPFDKAAADAA
ISVKRDLMQKGTPIGNNDTAIAGHALATGCVLVTNNTREFQRVSGLKLEDWSNRE

Nucleotide

• Length: 408 bp

>T158051 NZ_CP069559:c1244781-1243888 [Escherichia coli]
ATGCCTGAATTAACATCATTATTTCCTGACGTTGCCGATGCACTGGATATTGAATCTGCCGCTATCGTCGAAAAAGATTA
TTTTGTGGTTGATCTTTTAAGATTATTAAAAGAAATAAAACCAGAAACACATACTCTCGTGTTTGCAGGTAGTACCGCAT
TATCAAAAGCAGGTATCTCGCTAAACAGAATGTCAGAAGATATTGATATAAAGCTCGTGCCAACAGAGAACTTTATGCAA
AATGGCAGAGACAAGAGAAGAAAAATTCGCAAGGAAATCGTGCAAATAATCACGGATGTTATTCATAATTCTGACATTTT
CAGCTTGGATAATGAAAACGCCCGTATAACACGTGATGAGTATCGTTATAACGAAATCTCGGTACGTTATCCCCAGACAT
TTGCACAAGTCCCCTGTCTTCGTCCATTTATTAAGCTGGAGTTGATGGAAAGCACTCTGCTGGAACATCCGGAAAGCAGA
GATATTTATTCCCTTGTAACGGAGTTAACGGGTAAAGGTACACCTATAACAGCATTCCCCTGTGCCACCATTTTATCCAC
TCAGGCTGAAAAGCTCATCTCCATGATGCGAAGAACAGCAGCACATCTTCGTAATCCCGAACAACAGGATGATGAGTTTC
TGGTACGTCACATTTATGACAACTACTGCATTGTCAGGGAAAAAGGTGTGAATGTTCCTGTATTGAAAAATTTTGTTCAG
ATATGTATTCAACAGGATATTGAACGCTATGGTAACCAGTACCCACAATTTTGTAGCTCACCAGTTGATGAACTTAAGAA
AGGGCTGGATGCACTCCGGGATTATCCTGTACACAAATTGCGCTTTCAACGATTTGTTAACCCGATGGTATTTGGTAATA
CACAGATTAACTAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9138.11 Da
• Isoelectric Point: 4.7962

>AT158051 WP_175247819.1 NZ_CP053818:c23932-23696 [Vibrio cholerae]
MRRTVSIFRNGSNQSVRLPKDFEFSGVNELEIQKEGDVITLRPARPDWLSFSQHEKADSDFMAQRNDVMNDEGRFNFE

Nucleotide

• Length: 237 bp

>AT158051 NZ_CP069559:c1245169-1244774 [Escherichia coli]
ATGACTACAAAAGACCGTATTCAGTCCCGACTGAACCGTTCAAAACGTTATGTGTTCACCCGGGATGATTTCAGGGATAT
CGCTGGCTATGATCAGGTTGGCAGAGCTCTTCGTACACTGGTAAACGAAGGTAAGTTGATGAAAGTGGGCTATGGTGTTT
ACACCAAAGCCCGTCGTAACATCATCACTGGGAAAATCATGCCCGCAGCGCCTGGGGGCTCTGCTGCAGTAATTACAGAG
GCCCTGGAGCGCCTGAATATCAGATACCGTCTGGCAGGAGCAACGGAATGCTACAATAAAGGTCAGTCCACACAAATTCC
TGTATCTCTGGGGATAAAAACACCTTCTCGATTTAAAAGAGTGTTAATAGTAGGGAAAAGTAAAATCAATGCCTGA