Detailed information of TA system TA158044

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 471833..472331
• Replicon: chromosome
• Accession: NZ_CP053817
• Organism: Vibrio cholerae strain SA7G

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: HPY17_RS16195
• Protein ID: WP_000589156.1
• Coordinates: 471833..472099 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: HPY17_RS16200
• Protein ID: WP_000643598.1
• Coordinates: 472086..472331 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY17_RS16150	467298..467723	+	426	WP_000415750.1	hypothetical protein	-
HPY17_RS16155	468071..468252	+	182	Protein_522	DUF645 family protein	-
HPY17_RS16160	468744..468950	+	207	WP_175247674.1	DUF3265 domain-containing protein	-
HPY17_RS16165	468953..469273	+	321	WP_175247675.1	DUF1778 domain-containing protein	-
HPY17_RS16170	469270..469803	+	534	WP_175246444.1	GNAT family N-acetyltransferase	-
HPY17_RS16175	470109..470291	+	183	WP_001933694.1	DUF645 family protein	-
HPY17_RS16180	470440..471045	+	606	WP_175242423.1	suppressor of fused domain protein	-
HPY17_RS16185	471050..471160	+	111	WP_080100071.1	DUF3265 domain-containing protein	-
HPY17_RS16190	471201..471656	+	456	WP_000432865.1	hypothetical protein	-
HPY17_RS16195	471833..472099	+	267	WP_000589156.1	BrnT family toxin	Toxin
HPY17_RS16200	472086..472331	+	246	WP_000643598.1	hypothetical protein	Antitoxin
HPY17_RS16205	472806..472916	+	111	WP_086017781.1	DUF3265 domain-containing protein	-
HPY17_RS16210	472958..473368	+	411	WP_025613414.1	cytidine deaminase	-
HPY17_RS16215	473338..473475	+	138	WP_175246445.1	DUF3265 domain-containing protein	-
HPY17_RS16220	473512..474213	+	702	WP_001175917.1	hypothetical protein	-
HPY17_RS16225	474451..475854	+	1404	WP_175246446.1	AAA family ATPase	-
HPY17_RS16230	475847..476398	+	552	WP_175246447.1	hypothetical protein	-
HPY17_RS16235	476711..476892	+	182	Protein_538	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	310054..510566	200512
	-	inside	Integron	-	-	311218..510566	199348

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T158044 WP_000589156.1 NZ_CP053817:471833-472099 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T158044 NZ_CP069549:4405275-4405610 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT158044 WP_000643598.1 NZ_CP053817:472086-472331 [Vibrio cholerae]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT158044 NZ_CP069549:4405027-4405275 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: A0A271VME2