Detailed information of TA system TA158040

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc(toxin)
• Location: 442094..442665
• Replicon: chromosome
• Accession: NZ_CP053817
• Organism: Vibrio cholerae strain SA7G

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9KMA2
• Locus tag: HPY17_RS15950
• Protein ID: WP_000351248.1
• Coordinates: 442264..442665 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q8GNG3
• Locus tag: HPY17_RS15945
• Protein ID: WP_001080654.1
• Coordinates: 442094..442264 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY17_RS15910	438848..438944	+	97	Protein_473	DUF645 family protein	-
HPY17_RS15915	439300..439482	+	183	WP_071170650.1	DUF645 family protein	-
HPY17_RS15920	439688..440026	+	339	WP_175246331.1	hypothetical protein	-
HPY17_RS15925	440181..440546	+	366	WP_069730855.1	hypothetical protein	-
HPY17_RS15930	440692..441492	+	801	WP_114763308.1	nucleotide-binding protein	-
HPY17_RS15935	441483..441599	+	117	WP_114763309.1	DUF3265 domain-containing protein	-
HPY17_RS15940	441639..441914	+	276	WP_000436062.1	hypothetical protein	-
HPY17_RS15945	442094..442264	+	171	WP_001080654.1	hypothetical protein	Antitoxin
HPY17_RS15950	442264..442665	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
HPY17_RS15955	442652..442825	+	174	WP_175246605.1	DUF3265 domain-containing protein	-
HPY17_RS15960	442800..443288	+	489	WP_044128169.1	GNAT family N-acetyltransferase	-
HPY17_RS15965	443428..443706	-	279	WP_000578473.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
HPY17_RS15970	443703..443987	-	285	WP_000381186.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
HPY17_RS15975	444797..445804	+	1008	WP_175246433.1	hypothetical protein	-
HPY17_RS15980	445874..445993	+	120	WP_114780049.1	acetyltransferase	-
HPY17_RS15985	445934..446251	-	318	WP_148532547.1	CcdB family protein	-
HPY17_RS15990	446251..446496	-	246	WP_001260801.1	type II toxin-antitoxin system CcdA family antitoxin	-
HPY17_RS15995	446851..446994	+	144	Protein_490	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	310054..510566	200512
	-	inside	Integron	-	-	311218..510566	199348

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-132)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14608.71 Da
• Isoelectric Point: 4.0128

>T158040 WP_000351248.1 NZ_CP053817:442264-442665 [Vibrio cholerae]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Nucleotide

• Length: 402 bp

>T158040 NZ_CP069549:c2889140-2888967 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6287.35 Da
• Isoelectric Point: 10.7274

>AT158040 WP_001080654.1 NZ_CP053817:442094-442264 [Vibrio cholerae]
MNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 171 bp

>AT158040 NZ_CP069549:c2889464-2889168 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A0Q0PN34

Antitoxin

• AlphaFold DB: A0A1T4SJJ1