Detailed information of TA system TA158028

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 521176..521674
• Replicon: chromosome
• Accession: NZ_CP053814
• Organism: Vibrio cholerae strain W7G

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: HPY09_RS16495
• Protein ID: WP_000589156.1
• Coordinates: 521176..521442 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HPY09_RS16500
• Protein ID: WP_071180555.1
• Coordinates: 521429..521674 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY09_RS16460	516985..517410	+	426	WP_000415750.1	hypothetical protein	-
HPY09_RS16465	517694..518119	+	426	WP_071180452.1	hypothetical protein	-
HPY09_RS16470	518318..518731	+	414	WP_071180259.1	VOC family protein	-
HPY09_RS16475	519087..519218	+	132	WP_080483798.1	DUF645 family protein	-
HPY09_RS16480	519582..519752	+	171	WP_101411697.1	DUF645 family protein	-
HPY09_RS16485	519997..520403	+	407	Protein_600	VOC family protein	-
HPY09_RS16490	520551..520985	+	435	WP_057563745.1	GNAT family N-acetyltransferase	-
HPY09_RS16495	521176..521442	+	267	WP_000589156.1	BrnT family toxin	Toxin
HPY09_RS16500	521429..521674	+	246	WP_071180555.1	CopG family transcriptional regulator	Antitoxin
HPY09_RS16505	521817..522317	+	501	WP_000686370.1	NAD(P)H-dependent oxidoreductase	-
HPY09_RS16510	522589..522777	+	189	WP_080483779.1	DUF3709 domain-containing protein	-
HPY09_RS16515	522974..523933	-	960	Protein_606	IS30 family transposase	-
HPY09_RS16520	524219..524734	+	516	WP_000284464.1	hypothetical protein	-
HPY09_RS16525	524914..525084	+	171	WP_001080654.1	hypothetical protein	-
HPY09_RS16530	525084..525485	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
HPY09_RS16535	525758..525940	+	183	WP_000093413.1	DUF645 family protein	-
HPY09_RS16540	526137..526607	-	471	WP_000784954.1	RES family NAD+ phosphorylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	317876..527757	209881
	-	inside	IScluster/Tn	-	-	522974..528646	5672

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T158028 WP_000589156.1 NZ_CP053814:521176-521442 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T158028 NZ_CP069549:548592-548846 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9526.85 Da
• Isoelectric Point: 6.8803

>AT158028 WP_071180555.1 NZ_CP053814:521429-521674 [Vibrio cholerae]
MKAHEFDAKFESYDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT158028 NZ_CP069549:548344-548595 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A271VMP7