Detailed information of TA system TA158015

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2215661..2217486
• Replicon: chromosome
• Accession: NZ_CP053813
• Organism: Vibrio cholerae strain W7G

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: HPY09_RS10000
• Protein ID: WP_142993673.1
• Coordinates: 2215661..2217022 (+)
• Length: 454 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HPY09_RS10005
• Protein ID: WP_000804846.1
• Coordinates: 2217019..2217486 (+)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY09_RS09970	2211196..2212860	+	1665	WP_175246987.1	DNA repair protein RecN	-
HPY09_RS09975	2213019..2213378	+	360	WP_001160907.1	outer membrane protein assembly factor BamE	-
HPY09_RS09980	2213454..2213759	-	306	WP_001918351.1	RnfH family protein	-
HPY09_RS09985	2213762..2214196	-	435	WP_000815880.1	type II toxin-antitoxin system RatA family toxin	-
HPY09_RS09990	2214380..2214865	+	486	WP_000162689.1	SsrA-binding protein SmpB	-
HPY09_RS10000	2215661..2217022	+	1362	WP_142993673.1	type II toxin-antitoxin system HipA family toxin	Toxin
HPY09_RS10005	2217019..2217486	+	468	WP_000804846.1	helix-turn-helix domain-containing protein	Antitoxin
HPY09_RS10010	2218078..2220111	+	2034	WP_095461558.1	DNA phosphorothioation-associated putative methyltransferase	-
HPY09_RS10015	2220108..2220911	-	804	WP_071180710.1	restriction endonuclease	-
HPY09_RS10020	2220918..2222042	-	1125	WP_000214766.1	cysteine desulfurase DndA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-385)

Antitoxin

• Cro/C1-type helix-turn-helix domain (80-138)

Sequences

Toxin

Protein

• Length: 454 a.a.
• Molecular weight: 51331.23 Da
• Isoelectric Point: 4.8586

>T158015 WP_142993673.1 NZ_CP053813:2215661-2217022 [Vibrio cholerae]
MEELTVQAFIRGEWIDIGIISFPKSSQHNFRVTELNYLSDYALEHYDKDDFHAVSLNHPVSFIFDDMGKPGWLKFLDDIM
PSGASRRYWVKHLDIEDLSSDEQDYVLLKFGTMSPIGNLRIKDSLPERYEVADNLYFSVDDVKNRAGDFLDYAQQRGAAA
GGATGAGGEAPKLILRCGFDHGSGSEKIWIDPYQDDNSNHDLHYLVKYPRGSRSTIDCNILRAEFYFYHELTEMGVETIS
TDGMRLEEGLNYPSLWLPRFDVQIIDQQIERFGMESVYSILNKGAGVTLDHETTIRTLIEKITESNMVKHQGYRFDTQAF
VIEWVKRDLLNILFGNSDNHGRNTSFLKGDGVIKLAPIYDFAPMKADPEGIPRTTKWKAPLEVGGTYDFVGIADTLSDLV
PKEMLLKELAITASKCVGLKQRLALRGVPEQILEMPAVGLNYLSEKLTKWGLL

Nucleotide

• Length: 1362 bp

>T158015 NZ_CP069542:c2934882-2934706 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17047.52 Da
• Isoelectric Point: 10.7084

>AT158015 WP_000804846.1 NZ_CP053813:2217019-2217486 [Vibrio cholerae]
MKPSNKESVQDTVARLRANRNQQQPKVGKNATHSSKEQTPLDTPTRSKASKNAKAVSAAERKTEANKIIKHLLLGELTQG
QALKSLRINILGLKQDVFARLVDVSRKTLSDIENDRGSYNTEILNKVFKPFSLKIGLLPSSPDALKSLLMDGEDG

Nucleotide

• Length: 468 bp

>AT158015 NZ_CP069542:c2934660-2934244 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA