Detailed information of TA system TA158012

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc(toxin)
• Location: 524891..525462
• Replicon: chromosome
• Accession: NZ_CP053811
• Organism: Vibrio cholerae strain W6G

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9KMA2
• Locus tag: HPY05_RS16535
• Protein ID: WP_000351248.1
• Coordinates: 525061..525462 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q8GNG3
• Locus tag: HPY05_RS16530
• Protein ID: WP_001080654.1
• Coordinates: 524891..525061 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY05_RS16490	519974..520380	+	407	Protein_602	VOC family protein	-
HPY05_RS16495	520528..520962	+	435	WP_057563745.1	GNAT family N-acetyltransferase	-
HPY05_RS16500	521153..521419	+	267	WP_000589156.1	BrnT family toxin	-
HPY05_RS16505	521406..521651	+	246	WP_071180555.1	CopG family transcriptional regulator	-
HPY05_RS16510	521794..522294	+	501	WP_000686370.1	NAD(P)H-dependent oxidoreductase	-
HPY05_RS16515	522566..522754	+	189	WP_080483779.1	DUF3709 domain-containing protein	-
HPY05_RS16520	522951..523910	-	960	Protein_608	IS30 family transposase	-
HPY05_RS16525	524196..524711	+	516	WP_000284464.1	hypothetical protein	-
HPY05_RS16530	524891..525061	+	171	WP_001080654.1	hypothetical protein	Antitoxin
HPY05_RS16535	525061..525462	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
HPY05_RS16540	525735..525917	+	183	WP_000093413.1	DUF645 family protein	-
HPY05_RS16545	526114..526584	-	471	WP_000784954.1	RES family NAD+ phosphorylase	-
HPY05_RS16550	526584..527036	-	453	WP_000024295.1	DUF2384 domain-containing protein	-
HPY05_RS16555	527258..527734	+	477	WP_001047175.1	GNAT family N-acetyltransferase	-
HPY05_RS16560	528075..528202	+	128	Protein_616	DUF645 family protein	-
HPY05_RS16565	528189..528623	+	435	WP_095462747.1	IS200/IS605 family transposase	-
HPY05_RS16570	528702..529175	-	474	WP_000034756.1	FKBP-type peptidyl-prolyl cis-trans isomerase	-
HPY05_RS16575	529397..530011	+	615	WP_162808162.1	OmpA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	317868..527734	209866
	-	inside	IScluster/Tn	-	-	522951..528623	5672

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-132)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14608.71 Da
• Isoelectric Point: 4.0128

>T158012 WP_000351248.1 NZ_CP053811:525061-525462 [Vibrio cholerae]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Nucleotide

• Length: 402 bp

>T158012 NZ_CP069542:2527247-2527350 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6287.35 Da
• Isoelectric Point: 10.7274

>AT158012 WP_001080654.1 NZ_CP053811:524891-525061 [Vibrio cholerae]
MNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 171 bp

>AT158012 NZ_CP069542:c2527388-2527117 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A0Q0PN34

Antitoxin

• AlphaFold DB: A0A1T4SJJ1