Detailed information of TA system TA158011

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 521153..521651
• Replicon: chromosome
• Accession: NZ_CP053811
• Organism: Vibrio cholerae strain W6G

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: HPY05_RS16500
• Protein ID: WP_000589156.1
• Coordinates: 521153..521419 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HPY05_RS16505
• Protein ID: WP_071180555.1
• Coordinates: 521406..521651 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY05_RS16465	516962..517387	+	426	WP_000415750.1	hypothetical protein	-
HPY05_RS16470	517671..518096	+	426	WP_071180452.1	hypothetical protein	-
HPY05_RS16475	518295..518708	+	414	WP_071180259.1	VOC family protein	-
HPY05_RS16480	519064..519195	+	132	WP_080483798.1	DUF645 family protein	-
HPY05_RS16485	519559..519729	+	171	WP_101411697.1	DUF645 family protein	-
HPY05_RS16490	519974..520380	+	407	Protein_602	VOC family protein	-
HPY05_RS16495	520528..520962	+	435	WP_057563745.1	GNAT family N-acetyltransferase	-
HPY05_RS16500	521153..521419	+	267	WP_000589156.1	BrnT family toxin	Toxin
HPY05_RS16505	521406..521651	+	246	WP_071180555.1	CopG family transcriptional regulator	Antitoxin
HPY05_RS16510	521794..522294	+	501	WP_000686370.1	NAD(P)H-dependent oxidoreductase	-
HPY05_RS16515	522566..522754	+	189	WP_080483779.1	DUF3709 domain-containing protein	-
HPY05_RS16520	522951..523910	-	960	Protein_608	IS30 family transposase	-
HPY05_RS16525	524196..524711	+	516	WP_000284464.1	hypothetical protein	-
HPY05_RS16530	524891..525061	+	171	WP_001080654.1	hypothetical protein	-
HPY05_RS16535	525061..525462	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
HPY05_RS16540	525735..525917	+	183	WP_000093413.1	DUF645 family protein	-
HPY05_RS16545	526114..526584	-	471	WP_000784954.1	RES family NAD+ phosphorylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	317868..527734	209866
	-	inside	IScluster/Tn	-	-	522951..528623	5672

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T158011 WP_000589156.1 NZ_CP053811:521153-521419 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T158011 NZ_CP069542:c2314887-2314513 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9526.85 Da
• Isoelectric Point: 6.8803

>AT158011 WP_071180555.1 NZ_CP053811:521406-521651 [Vibrio cholerae]
MKAHEFDAKFESYDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT158011 NZ_CP069542:c2315344-2314976 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7