Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc(toxin)
Location 517611..518182 Replicon chromosome
Accession NZ_CP053809
Organism Vibrio cholerae strain SP7G

Toxin (Protein)


Gene name doc Uniprot ID Q9KMA2
Locus tag HPY08_RS15810 Protein ID WP_000351248.1
Coordinates 517781..518182 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID Q8GNG3
Locus tag HPY08_RS15805 Protein ID WP_001080654.1
Coordinates 517611..517781 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPY08_RS15770 513114..513827 + 714 WP_000123660.1 Fic family protein -
HPY08_RS15775 514189..514299 + 111 Protein_569 DUF645 family protein -
HPY08_RS15780 514503..514841 + 339 WP_046127316.1 hypothetical protein -
HPY08_RS15785 514882..515841 - 960 WP_001107099.1 IS30 family transposase -
HPY08_RS15790 516156..516443 + 288 WP_001162670.1 type II toxin-antitoxin system RelE/ParE family toxin -
HPY08_RS15795 516454..516771 + 318 WP_001232701.1 HigA family addiction module antidote protein -
HPY08_RS15800 516914..517432 + 519 WP_114708872.1 GNAT family N-acetyltransferase -
HPY08_RS15805 517611..517781 + 171 WP_001080654.1 hypothetical protein Antitoxin
HPY08_RS15810 517781..518182 + 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
HPY08_RS15815 518455..518637 + 183 WP_000093413.1 DUF645 family protein -
HPY08_RS15820 518831..519301 - 471 WP_000784954.1 RES family NAD+ phosphorylase -
HPY08_RS15825 519301..519753 - 453 WP_000024295.1 DUF2384 domain-containing protein -
HPY08_RS15830 519974..520450 + 477 WP_114708866.1 GNAT family N-acetyltransferase -
HPY08_RS15835 520791..520918 + 128 Protein_581 DUF645 family protein -
HPY08_RS15840 520905..521339 + 435 WP_001885561.1 IS200/IS605 family transposase -
HPY08_RS15845 521418..521891 - 474 WP_000034756.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
HPY08_RS15850 522113..522727 + 615 WP_162797168.1 OmpA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 338606..521339 182733
- inside Integron - - 339770..520450 180680
- flank IS/Tn - - 520791..521339 548


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(1-132)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14608.71 Da        Isoelectric Point: 4.0128

>T157993 WP_000351248.1 NZ_CP053809:517781-518182 [Vibrio cholerae]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Download         Length: 402 bp

>T157993 NZ_CP069530:18755-18904 [Escherichia coli]
ATGACGAAATACACCCTTATAGGGTTGCTCGCTGTGTGTGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAGCGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGATGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6287.35 Da        Isoelectric Point: 10.7274

>AT157993 WP_001080654.1 NZ_CP053809:517611-517781 [Vibrio cholerae]
MNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Download         Length: 171 bp

>AT157993 NZ_CP069530:c18710-18651 [Escherichia coli]
TAAGTACATCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0PN34

Antitoxin

Source ID Structure
AlphaFold DB A0A1T4SJJ1

References