Detailed information of TA system TA157981

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/YoeB-RelB
• Location: 451459..451969
• Replicon: chromosome
• Accession: NZ_CP053809
• Organism: Vibrio cholerae strain SP7G

Toxin (Protein)

• Gene name: relB
• Uniprot ID: A0A7Z7VK37
• Locus tag: HPY08_RS15265
• Protein ID: WP_000098715.1
• Coordinates: 451706..451969 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A7Y3QZ95
• Locus tag: HPY08_RS15260
• Protein ID: WP_001223794.1
• Coordinates: 451459..451713 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY08_RS15225	446903..447592	+	690	WP_114741983.1	FMN-binding negative transcriptional regulator	-
HPY08_RS15230	447910..448065	+	156	WP_108911962.1	DUF645 family protein	-
HPY08_RS15235	448420..448573	+	154	Protein_461	DUF645 family protein	-
HPY08_RS15240	448889..449314	+	426	WP_000415750.1	hypothetical protein	-
HPY08_RS15245	449486..449941	+	456	WP_108248536.1	GNAT family N-acetyltransferase	-
HPY08_RS15250	450208..450444	+	237	WP_175242888.1	DUF3709 domain-containing protein	-
HPY08_RS15255	450667..451248	+	582	WP_175242889.1	nucleotidyltransferase family protein	-
HPY08_RS15260	451459..451713	+	255	WP_001223794.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
HPY08_RS15265	451706..451969	+	264	WP_000098715.1	Txe/YoeB family addiction module toxin	Toxin
HPY08_RS15270	452178..453833	+	1656	WP_061051387.1	DUF262 domain-containing HNH endonuclease family protein	-
HPY08_RS15275	454028..454351	+	324	WP_080299109.1	DUF3709 domain-containing protein	-
HPY08_RS15280	454784..455173	+	390	WP_000491261.1	VOC family protein	-
HPY08_RS15285	455328..455825	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
HPY08_RS15290	455822..456094	-	273	WP_001915058.1	DUF1778 domain-containing protein	-
HPY08_RS15295	456430..456666	+	237	WP_002051081.1	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	338606..521339	182733
	-	inside	Integron	-	-	339770..520450	180680

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10415.75 Da
• Isoelectric Point: 7.9816

>T157981 WP_000098715.1 NZ_CP053809:451706-451969 [Vibrio cholerae]
MSSRLLSWTDESWDDYLYWQTQDKKTLKRINKIINDVKRSPFEGIGKPEPLKENLSGFWSRRIDDTNRLVYAVDDNAITI
ISCRYHY

Nucleotide

• Length: 264 bp

>T157981 NZ_CP069528:c3321578-3321264 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9437.64 Da
• Isoelectric Point: 5.0481

>AT157981 WP_001223794.1 NZ_CP053809:451459-451713 [Vibrio cholerae]
MRIVSFTEARNSLKAVLDAVVNDADTTVITRRDSEDAVVMSLDYYNSLMETVHLLRSPANAEHLNRSIAQFKAGKVTQRD
LIDE

Nucleotide

• Length: 255 bp

>AT157981 NZ_CP069528:c3321814-3321581 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: A0A7Z7VK37

Antitoxin

• AlphaFold DB: A0A7Y3QZ95