Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 377711..378477 Replicon chromosome
Accession NZ_CP053809
Organism Vibrio cholerae strain SP7G

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag HPY08_RS14650 Protein ID WP_000982260.1
Coordinates 377711..378208 (-) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9K2J6
Locus tag HPY08_RS14655 Protein ID WP_000246253.1
Coordinates 378205..378477 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPY08_RS14620 373352..373765 + 414 WP_000049417.1 VOC family protein -
HPY08_RS14625 373934..374854 + 921 WP_175242856.1 hypothetical protein -
HPY08_RS14630 375006..375449 + 444 WP_175242857.1 hypothetical protein -
HPY08_RS14635 375750..375932 + 183 WP_001907633.1 DUF645 family protein -
HPY08_RS14640 376113..376961 + 849 WP_175242858.1 hypothetical protein -
HPY08_RS14645 377146..377559 + 414 WP_000049417.1 VOC family protein -
HPY08_RS14650 377711..378208 - 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
HPY08_RS14655 378205..378477 - 273 WP_000246253.1 DUF1778 domain-containing protein Antitoxin
HPY08_RS14660 378700..379146 + 447 WP_001919130.1 GNAT family N-acetyltransferase -
HPY08_RS14665 379468..379620 + 153 WP_001884520.1 DUF645 family protein -
HPY08_RS14670 379827..380279 + 453 WP_000863106.1 GNAT family N-acetyltransferase -
HPY08_RS14675 381193..381516 + 324 WP_174500497.1 DUF3709 domain-containing protein -
HPY08_RS14680 381965..382120 + 156 WP_108911962.1 DUF645 family protein -
HPY08_RS14685 382338..383135 + 798 WP_042988539.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 338606..521339 182733
- inside Integron - - 339770..520450 180680


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T157976 WP_000982260.1 NZ_CP053809:c378208-377711 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T157976 NZ_CP069528:c2076958-2076856 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9683.22 Da        Isoelectric Point: 6.2988

>AT157976 WP_000246253.1 NZ_CP053809:c378477-378205 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT157976 NZ_CP069528:2076818-2077086 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
PDB 1Y9B
AlphaFold DB A0A0F2IC79

References