Detailed information of TA system TA157974

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 344721..345261
• Replicon: chromosome
• Accession: NZ_CP053809
• Organism: Vibrio cholerae strain SP7G

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A151JGK8
• Locus tag: HPY08_RS14335
• Protein ID: WP_000277238.1
• Coordinates: 344721..344990 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q9KML3
• Locus tag: HPY08_RS14340
• Protein ID: WP_001258569.1
• Coordinates: 344983..345261 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY08_RS14295	339770..340732	-	963	WP_000841998.1	integron integrase IntIA	-
HPY08_RS14300	341138..341328	+	191	Protein_274	DUF645 family protein	-
HPY08_RS14305	341579..342004	+	426	WP_032473260.1	hypothetical protein	-
HPY08_RS14310	342696..343529	+	834	WP_175242835.1	hypothetical protein	-
HPY08_RS14315	343526..343675	+	150	WP_175243014.1	DUF3265 domain-containing protein	-
HPY08_RS14320	343688..343999	+	312	WP_000623749.1	hypothetical protein	-
HPY08_RS14325	344188..344586	+	399	WP_095488424.1	hypothetical protein	-
HPY08_RS14330	344550..344693	+	144	WP_175243015.1	DUF3265 domain-containing protein	-
HPY08_RS14335	344721..344990	-	270	WP_000277238.1	type II toxin-antitoxin system YafQ family toxin	Toxin
HPY08_RS14340	344983..345261	-	279	WP_001258569.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HPY08_RS14345	345624..345797	+	174	WP_114716423.1	DUF645 family protein	-
HPY08_RS14350	346060..346233	-	174	WP_175242836.1	hypothetical protein	-
HPY08_RS14355	346534..346791	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
HPY08_RS14360	346779..347081	+	303	WP_061900969.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HPY08_RS14365	347091..347207	+	117	Protein_287	DUF3265 domain-containing protein	-
HPY08_RS14370	347241..347729	+	489	WP_175242837.1	hypothetical protein	-
HPY08_RS14375	347864..348328	+	465	WP_175242838.1	N-acetyltransferase	-
HPY08_RS14380	348397..348486	+	90	WP_175242839.1	acetyltransferase	-
HPY08_RS14385	348486..348776	+	291	WP_175242840.1	hypothetical protein	-
HPY08_RS14390	348849..349313	-	465	WP_175242841.1	hypothetical protein	-
HPY08_RS14395	349814..349984	+	171	WP_162298991.1	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	338606..521339	182733
	-	inside	Integron	-	-	339770..520450	180680

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (2-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10406.11 Da
• Isoelectric Point: 9.5084

>T157974 WP_000277238.1 NZ_CP053809:c344990-344721 [Vibrio cholerae]
MYKLEYSTQFKKDFKKITKMPISDIIEVGNVISKLQRGEKLEPKNVDHPLTGNWVGFRDCHIKPDLVLIYRVFNDQLQLA
RIGSHSDLF

Nucleotide

• Length: 270 bp

>T157974 NZ_CP069528:c1468409-1468302 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10088.58 Da
• Isoelectric Point: 6.0728

>AT157974 WP_001258569.1 NZ_CP053809:c345261-344983 [Vibrio cholerae]
MRTEMLSTRIDHDTKIAFTNVCDEMGLSTSQAIKLFAKAVINHGGIPFELRVPQPNEVTASAIQELVEGKGHKAESVEAM
LNELTEGKVKHV

Nucleotide

• Length: 279 bp

>AT157974 NZ_CP069528:1468462-1468523 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A151JGK8

Antitoxin

• AlphaFold DB: A0A151KTP9