Detailed information of TA system TA157973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2123424..2125246
• Replicon: chromosome
• Accession: NZ_CP053808
• Organism: Vibrio cholerae strain SP7G

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: HPY08_RS09465
• Protein ID: WP_162797150.1
• Coordinates: 2123424..2124785 (+)
• Length: 454 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HPY08_RS09470
• Protein ID: WP_162797151.1
• Coordinates: 2124782..2125246 (+)
• Length: 155 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY08_RS09435	2118958..2120622	+	1665	WP_095463275.1	DNA repair protein RecN	-
HPY08_RS09440	2120781..2121140	+	360	WP_001160907.1	outer membrane protein assembly factor BamE	-
HPY08_RS09445	2121217..2121522	-	306	WP_001929603.1	RnfH family protein	-
HPY08_RS09450	2121525..2121959	-	435	WP_000815880.1	type II toxin-antitoxin system RatA family toxin	-
HPY08_RS09455	2122143..2122628	+	486	WP_000162689.1	SsrA-binding protein SmpB	-
HPY08_RS09465	2123424..2124785	+	1362	WP_162797150.1	type II toxin-antitoxin system HipA family toxin	Toxin
HPY08_RS09470	2124782..2125246	+	465	WP_162797151.1	helix-turn-helix domain-containing protein	Antitoxin
HPY08_RS09475	2125674..2126252	+	579	WP_000109982.1	class I SAM-dependent methyltransferase	-
HPY08_RS09480	2126256..2129177	+	2922	WP_114717083.1	DEAD/DEAH box helicase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-385)

Antitoxin

• Cro/C1-type helix-turn-helix domain (79-137)

Sequences

Toxin

Protein

• Length: 454 a.a.
• Molecular weight: 51308.19 Da
• Isoelectric Point: 4.8042

>T157973 WP_162797150.1 NZ_CP053808:2123424-2124785 [Vibrio cholerae]
MEELTVQAFIRGEWIDIGIISFPKSSQHNFRVTELNYLSDYALEHYDKDDFHAVSLNHPVSFIFDDMGKPGWLKFLDDIM
PSGASRRYWVKNLDIEDLSSDEQDYVLLKFGTMSPIGNLRIKDSLPERYEVADNLYFSVDDVKNRAGDFLDYAQQRGAAA
GGATGAGGEAPKLILRCGFDHGSGSEKIWIDPYQDDNSNHDLHYLVKYPRGSRSTIDCNILRAEFYFYHELTEMGVETIS
TDGMRLEEGLNYPSLWLPRFDVQIIDQQIERFGMESVYSILNKGAGVTLDHETTIRTLIEKITESNMVKHQGYRFDTQAF
VIEWVKRDLLNILFGNSDNHGRNTSFLKGDGVIKLAPIYDFAPMKADPEGIPRTTKWKAPLEVGGTYDFVGIADTLSDLV
PKEMLLKELAITASKCVGLKQRLALRGVPEQILEMPAVGLNYLSEKLTKWGLL

Nucleotide

• Length: 1362 bp

>T157973 NZ_CP069528:c1042412-1042038 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 155 a.a.
• Molecular weight: 16976.48 Da
• Isoelectric Point: 10.7084

>AT157973 WP_162797151.1 NZ_CP053808:2124782-2125246 [Vibrio cholerae]
MKPSNKESVQDTVARLRANRNQQQPKVGKNATHSIKEQTLDTPTRSKASKNAKAVSAAERKTEANKIIKHLLLGELTQGQ
ALKSLRINILGLKQDVFARLVDVSRKTLSDIENDRGSYNTEILNKVFKPFSLKIGLLPSSPDALKSLLMDGEDG

Nucleotide

• Length: 465 bp

>AT157973 NZ_CP069528:c1042869-1042501 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGTTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA