Detailed information of TA system TA157958

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 347498..347996
• Replicon: chromosome
• Accession: NZ_CP053807
• Organism: Vibrio cholerae strain SP6G

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: HPY07_RS14950
• Protein ID: WP_000589156.1
• Coordinates: 347498..347764 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: HPY07_RS14955
• Protein ID: WP_000643598.1
• Coordinates: 347751..347996 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY07_RS14915	343453..343701	+	249	WP_175242420.1	DUF3709 domain-containing protein	-
HPY07_RS14920	343982..344509	+	528	WP_100692775.1	hypothetical protein	-
HPY07_RS14925	344527..344616	+	90	WP_175242421.1	DUF3265 domain-containing protein	-
HPY07_RS14930	344647..345282	+	636	WP_000691786.1	DUF2807 domain-containing protein	-
HPY07_RS14935	345416..345802	+	387	Protein_314	DUF3709 domain-containing protein	-
HPY07_RS14940	346073..346894	+	822	WP_175242281.1	HNH endonuclease	-
HPY07_RS14945	347051..347272	+	222	WP_069213624.1	hypothetical protein	-
HPY07_RS14950	347498..347764	+	267	WP_000589156.1	BrnT family toxin	Toxin
HPY07_RS14955	347751..347996	+	246	WP_000643598.1	hypothetical protein	Antitoxin
HPY07_RS14960	348137..348646	+	510	WP_114777038.1	AAA family ATPase	-
HPY07_RS14965	348675..348764	+	90	WP_175242282.1	hypothetical protein	-
HPY07_RS14970	348937..349119	+	183	WP_114763558.1	DUF645 family protein	-
HPY07_RS14975	349424..349660	+	237	WP_001065132.1	hypothetical protein	-
HPY07_RS14980	349892..350152	-	261	WP_108336272.1	hypothetical protein	-
HPY07_RS14985	350202..350444	-	243	WP_108336273.1	hypothetical protein	-
HPY07_RS14990	350881..351123	+	243	WP_175242283.1	hypothetical protein	-
HPY07_RS14995	351297..351572	+	276	WP_000436062.1	hypothetical protein	-
HPY07_RS15000	351716..352213	+	498	WP_108315890.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	309110..469370	160260
	-	inside	Integron	-	-	309781..469370	159589

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T157958 WP_000589156.1 NZ_CP053807:347498-347764 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T157958 NZ_CP069522:c4154077-4153763 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT157958 WP_000643598.1 NZ_CP053807:347751-347996 [Vibrio cholerae]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT157958 NZ_CP069522:c4154313-4154080 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: A0A271VME2