Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /BrnT(toxin)
Location 331065..331563 Replicon chromosome
Accession NZ_CP053805
Organism Vibrio cholerae strain SL6Y

Toxin (Protein)


Gene name - Uniprot ID Q9KMK7
Locus tag HPY06_RS14610 Protein ID WP_000589156.1
Coordinates 331065..331331 (+) Length 89 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9KMK6
Locus tag HPY06_RS14615 Protein ID WP_000643598.1
Coordinates 331318..331563 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPY06_RS14580 326726..327409 + 684 WP_102787193.1 hypothetical protein -
HPY06_RS14585 327593..328234 + 642 WP_175231339.1 FMN-binding negative transcriptional regulator -
HPY06_RS14590 328373..328726 + 354 WP_175231342.1 glyoxalase/bleomycin resistance/dioxygenase family protein -
HPY06_RS14595 328723..328833 + 111 WP_175231855.1 DUF3265 domain-containing protein -
HPY06_RS14600 329344..330354 + 1011 WP_175231345.1 hypothetical protein -
HPY06_RS14605 330648..330830 + 183 WP_057570823.1 DUF645 family protein -
HPY06_RS14610 331065..331331 + 267 WP_000589156.1 BrnT family toxin Toxin
HPY06_RS14615 331318..331563 + 246 WP_000643598.1 hypothetical protein Antitoxin
HPY06_RS14620 331953..332045 + 93 Protein_290 DUF645 family protein -
HPY06_RS14625 332244..332684 + 441 WP_114774848.1 hypothetical protein -
HPY06_RS14630 332919..333410 + 492 WP_069648535.1 hypothetical protein -
HPY06_RS14635 333545..333967 + 423 WP_099612058.1 hypothetical protein -
HPY06_RS14640 334258..334411 + 154 Protein_294 DUF645 family protein -
HPY06_RS14645 334628..335320 + 693 Protein_295 hypothetical protein -
HPY06_RS14650 335490..336557 + 1068 WP_175231348.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 308588..403342 94754
- inside Integron - - 309752..402202 92450


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(6-81)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10092.34 Da        Isoelectric Point: 7.3171

>T157940 WP_000589156.1 NZ_CP053805:331065-331331 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Download         Length: 267 bp

>T157940 NZ_CP069517:4818330-4818644 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9478.77 Da        Isoelectric Point: 6.4832

>AT157940 WP_000643598.1 NZ_CP053805:331318-331563 [Vibrio cholerae]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Download         Length: 246 bp

>AT157940 NZ_CP069517:4818094-4818327 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A271VMP7

Antitoxin

Source ID Structure
AlphaFold DB A0A271VME2

References