Detailed information of TA system TA157936

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc(toxin)
• Location: 507707..508278
• Replicon: chromosome
• Accession: NZ_CP053803
• Organism: Vibrio cholerae strain L6G

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: HPY16_RS15830
• Protein ID: WP_134987537.1
• Coordinates: 507877..508278 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q8GNG3
• Locus tag: HPY16_RS15825
• Protein ID: WP_001080654.1
• Coordinates: 507707..507877 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY16_RS15795	503034..503462	+	429	WP_000082746.1	hypothetical protein	-
HPY16_RS15800	504156..505073	+	918	WP_032467917.1	alpha/beta hydrolase	-
HPY16_RS15805	505445..505555	+	111	WP_076025930.1	DUF645 family protein	-
HPY16_RS15810	506289..506576	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HPY16_RS15815	506587..506904	+	318	WP_001232701.1	HigA family addiction module antidote protein	-
HPY16_RS15820	507110..507547	+	438	WP_000503164.1	IS200/IS605-like element IS1004 family transposase	-
HPY16_RS15825	507707..507877	+	171	WP_001080654.1	hypothetical protein	Antitoxin
HPY16_RS15830	507877..508278	+	402	WP_134987537.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
HPY16_RS15835	508551..508733	+	183	WP_000093413.1	DUF645 family protein	-
HPY16_RS15840	508931..509407	+	477	WP_085604293.1	GNAT family N-acetyltransferase	-
HPY16_RS15845	509748..509908	+	161	Protein_615	DUF645 family protein	-
HPY16_RS15850	509862..510278	+	417	WP_134987692.1	IS200/IS605 family transposase	-
HPY16_RS15855	510374..510847	-	474	WP_000034756.1	FKBP-type peptidyl-prolyl cis-trans isomerase	-
HPY16_RS15860	511069..511683	+	615	WP_002054141.1	OmpA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	blaCARB-7	-	309435..510278	200843
	-	inside	Integron	-	-	310548..509407	198859
	-	inside	IScluster/Tn	-	-	507110..510278	3168

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-132)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14651.74 Da
• Isoelectric Point: 4.0904

>T157936 WP_134987537.1 NZ_CP053803:507877-508278 [Vibrio cholerae]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHARPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Nucleotide

• Length: 402 bp

>T157936 NZ_CP069517:c4628057-4627800 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6287.35 Da
• Isoelectric Point: 10.7274

>AT157936 WP_001080654.1 NZ_CP053803:507707-507877 [Vibrio cholerae]
MNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 171 bp

>AT157936 NZ_CP069517:c4627788-4627237 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCGACCTTCAAAGGAGAAATGGATTCACCGCTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A1T4SJJ1