Detailed information of TA system TA157921

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higB-phd/HigB(toxin)
• Location: 324365..324815
• Replicon: chromosome
• Accession: NZ_CP053803
• Organism: Vibrio cholerae strain L6G

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: HPY16_RS14180
• Protein ID: WP_001162670.1
• Coordinates: 324528..324815 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: HPY16_RS14175
• Protein ID: WP_175248390.1
• Coordinates: 324365..324487 (+)
• Length: 41 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY16_RS14130	319883..320049	+	167	Protein_272	DUF645 family protein	-
HPY16_RS14135	320407..320535	+	129	WP_080551848.1	DUF645 family protein	-
HPY16_RS14140	320551..320589	+	39	WP_113629368.1	hypothetical protein	-
HPY16_RS14145	320788..321330	+	543	WP_142738652.1	yfeABCD locus regulator	-
HPY16_RS14150	321637..321818	+	182	Protein_276	DUF645 family protein	-
HPY16_RS14155	322014..322460	+	447	WP_000006157.1	hypothetical protein	-
HPY16_RS14160	322608..323123	+	516	WP_175248388.1	ClbS/DfsB family four-helix bundle protein	-
HPY16_RS14165	323193..323255	+	63	Protein_279	acetyltransferase	-
HPY16_RS14170	324067..324249	+	183	WP_175248389.1	DUF645 family protein	-
HPY16_RS14175	324365..324487	+	123	WP_175248390.1	acetyltransferase	Antitoxin
HPY16_RS14180	324528..324815	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HPY16_RS14185	324826..325143	+	318	WP_001232701.1	HigA family addiction module antidote protein	-
HPY16_RS14190	325325..325996	+	672	WP_069648515.1	hypothetical protein	-
HPY16_RS14195	326147..326635	+	489	WP_175248391.1	hypothetical protein	-
HPY16_RS14200	326774..327418	+	645	WP_146779505.1	zinc ribbon domain-containing protein	-
HPY16_RS14205	327723..327899	+	177	WP_043881278.1	DUF645 family protein	-
HPY16_RS14210	328259..328435	+	177	WP_175248392.1	DUF645 family protein	-
HPY16_RS14215	328644..329582	+	939	WP_175248393.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	blaCARB-7	-	309435..510278	200843
	-	inside	Integron	-	-	310548..509407	198859

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T157921 WP_001162670.1 NZ_CP053803:324528-324815 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T157921 NZ_CP069517:c28707-28489 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 41 a.a.
• Molecular weight: 4750.90 Da
• Isoelectric Point: 11.9647

>AT157921 WP_175248390.1 NZ_CP053803:324365-324487 [Vibrio cholerae]
VVSVVVFEFRVMRCQPLRRALCTFLKSFLQIKLVHSVIMR

Nucleotide

• Length: 123 bp

>AT157921 NZ_CP069517:c29107-28733 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0F2HI36