Detailed information of TA system TA157897

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DhiAT/-
• Location: 450469..451253
• Replicon: chromosome
• Accession: NZ_CP053799
• Organism: Vibrio cholerae strain SL5Y

Toxin (Protein)

• Gene name: dhiT
• Uniprot ID: A0A086SLD6
• Locus tag: HPY14_RS15950
• Protein ID: WP_001114075.1
• Coordinates: 450469..450738 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: dhiA
• Uniprot ID: -
• Locus tag: HPY14_RS15955
• Protein ID: WP_094393031.1
• Coordinates: 450732..451253 (+)
• Length: 174 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY14_RS15925	445865..446362	+	498	WP_094393098.1	GNAT family N-acetyltransferase	-
HPY14_RS15930	446657..446839	+	183	Protein_498	DUF645 family protein	-
HPY14_RS15935	447120..448199	+	1080	WP_094393028.1	type I restriction enzyme HsdR N-terminal domain-containing protein	-
HPY14_RS15940	448390..449376	-	987	WP_094393029.1	sulfate ABC transporter substrate-binding protein	-
HPY14_RS15945	449638..450276	+	639	WP_094393030.1	HD domain-containing protein	-
HPY14_RS15950	450469..450738	+	270	WP_001114075.1	DUF4160 domain-containing protein	Toxin
HPY14_RS15955	450732..451253	+	522	WP_094393031.1	DUF2442 domain-containing protein	Antitoxin
HPY14_RS15960	451419..452159	+	741	WP_162298990.1	PhzF family phenazine biosynthesis protein	-
HPY14_RS15965	452454..452636	+	183	WP_094393107.1	DUF645 family protein	-
HPY14_RS15970	452834..453607	+	774	WP_094393066.1	aminoglycoside phosphotransferase family protein	-
HPY14_RS15975	453761..454114	+	354	WP_094393064.1	hypothetical protein	-
HPY14_RS15980	454098..454793	+	696	WP_128739199.1	hypothetical protein	-
HPY14_RS15985	454998..455261	+	264	WP_032473149.1	hypothetical protein	-
HPY14_RS15990	455487..455729	+	243	WP_033930503.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	307775..455729	147954
	-	inside	Integron	-	-	308939..456912	147973

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF4160 (9-74)

Antitoxin

• Uncharacterized protein HI1420 (1-173)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10310.94 Da
• Isoelectric Point: 6.6303

>T157897 WP_001114075.1 NZ_CP053799:450469-450738 [Vibrio cholerae]
MPEIDALLGLSFCLYFFDNKQHKLPHIHVKYGSYELIIAIETGECLEGYLPNKQRKRAENHILEHREQLMVMWNKAVNGE
NPGKLGDLC

Nucleotide

• Length: 270 bp

>T157897 NZ_CP069501:c4467535-4467260 [Escherichia coli]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 174 a.a.
• Molecular weight: 19455.10 Da
• Isoelectric Point: 6.7342

>AT157897 WP_094393031.1 NZ_CP053799:450732-451253 [Vibrio cholerae]
MLKVIDVDFVSDHTLELMFNDGYQGYADLSVYFKKTPFSEIKDFKRFSLTRDGSLNWDGNELSAATLRDITKGSQKSVEL
SFNVQEMEAVIKQASWESMMEGRPDILQAAIRSYVEQFGHGQVIAKAGIKSRTSAYRSLKPETTPNFGTLVQLGHAVIEL
AKDRTVANKEPRI

Nucleotide

• Length: 522 bp

>AT157897 NZ_CP069501:c4467263-4466904 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A086SLD6