Detailed information of TA system TA157868

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): dhiT-phd/-
• Location: 381189..381593
• Replicon: chromosome
• Accession: NZ_CP053795
• Organism: Vibrio cholerae strain W10G

Toxin (Protein)

• Gene name: dhiT
• Uniprot ID: A0A086SLD6
• Locus tag: HPY12_RS15785
• Protein ID: WP_001114075.1
• Coordinates: 381324..381593 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: HPY12_RS15780
• Protein ID: WP_099607150.1
• Coordinates: 381189..381293 (+)
• Length: 35 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY12_RS15740	376210..376581	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
HPY12_RS15745	376648..377073	+	426	WP_001882332.1	DUF1778 domain-containing protein	-
HPY12_RS15750	377070..377603	+	534	WP_000118351.1	GNAT family N-acetyltransferase	-
HPY12_RS15755	378289..378498	+	210	Protein_370	GNAT family N-acetyltransferase	-
HPY12_RS15760	378617..379054	+	438	WP_000503164.1	IS200/IS605-like element IS1004 family transposase	-
HPY12_RS15765	379118..379336	+	219	WP_071908318.1	GNAT family N-acetyltransferase	-
HPY12_RS15770	379511..380383	+	873	Protein_373	DUF3800 domain-containing protein	-
HPY12_RS15775	380533..381132	+	600	WP_001891245.1	glutathione S-transferase N-terminal domain-containing protein	-
HPY12_RS15780	381189..381293	+	105	WP_099607150.1	acetyltransferase	Antitoxin
HPY12_RS15785	381324..381593	+	270	WP_001114075.1	DUF4160 domain-containing protein	Toxin
HPY12_RS15790	381587..382108	+	522	WP_000921691.1	DUF2442 domain-containing protein	-
HPY12_RS15795	382407..382532	+	126	WP_001905700.1	DUF645 family protein	-
HPY12_RS15800	382783..383178	+	396	WP_001000868.1	hypothetical protein	-
HPY12_RS15805	383317..383595	-	279	WP_001897114.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
HPY12_RS15810	383592..383876	-	285	WP_000381183.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
HPY12_RS15815	384070..384585	+	516	WP_000343786.1	GNAT family N-acetyltransferase	-
HPY12_RS15820	384769..385182	+	414	WP_000049420.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	306166..386842	80676
	-	inside	Integron	-	-	307330..385669	78339
	-	inside	IScluster/Tn	-	-	374870..379054	4184

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF4160 (9-74)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10310.94 Da
• Isoelectric Point: 6.6303

>T157868 WP_001114075.1 NZ_CP053795:381324-381593 [Vibrio cholerae]
MPEIDALLGLSFCLYFFDNKQHKLPHIHVKYGSYELIIAIETGECLEGYLPNKQRKRAENHILEHREQLMVMWNKAVNGE
NPGKLGDLC

Nucleotide

• Length: 270 bp

>T157868 NZ_CP069500:c926055-925804 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCCCTGGAGGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCTGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 35 a.a.
• Molecular weight: 3991.79 Da
• Isoelectric Point: 9.2853

>AT157868 WP_099607150.1 NZ_CP053795:381189-381293 [Vibrio cholerae]
VVSVVVFEFSSMRCQPLRRALYAYRNCLLKDTLL

Nucleotide

• Length: 105 bp

>AT157868 NZ_CP069500:c925807-925553 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCAGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A086SLD6