Detailed information of TA system TA157867

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 376648..377603
• Replicon: chromosome
• Accession: NZ_CP053795
• Organism: Vibrio cholerae strain W10G

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KM94
• Locus tag: HPY12_RS15750
• Protein ID: WP_000118351.1
• Coordinates: 377070..377603 (+)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HPY12_RS15745
• Protein ID: WP_001882332.1
• Coordinates: 376648..377073 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY12_RS15690	372311..372568	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
HPY12_RS15695	372556..372858	+	303	WP_000229325.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HPY12_RS15700	373146..373370	+	225	WP_161571222.1	DUF3709 domain-containing protein	-
HPY12_RS15705	373811..373897	+	87	WP_134820423.1	DUF645 family protein	-
HPY12_RS15710	373897..373989	+	93	Protein_361	DUF645 family protein	-
HPY12_RS15715	374140..374808	+	669	WP_002033340.1	hypothetical protein	-
HPY12_RS15720	374870..375307	-	438	WP_000503164.1	IS200/IS605-like element IS1004 family transposase	-
HPY12_RS15725	375421..375543	+	123	WP_154591787.1	DUF3265 domain-containing protein	-
HPY12_RS15730	375649..375684	+	36	WP_172884631.1	hypothetical protein	-
HPY12_RS15735	375702..375962	+	261	WP_032468027.1	DUF3709 domain-containing protein	-
HPY12_RS15740	376210..376581	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
HPY12_RS15745	376648..377073	+	426	WP_001882332.1	DUF1778 domain-containing protein	Antitoxin
HPY12_RS15750	377070..377603	+	534	WP_000118351.1	GNAT family N-acetyltransferase	Toxin
HPY12_RS15755	378289..378498	+	210	Protein_370	GNAT family N-acetyltransferase	-
HPY12_RS15760	378617..379054	+	438	WP_000503164.1	IS200/IS605-like element IS1004 family transposase	-
HPY12_RS15765	379118..379336	+	219	WP_071908318.1	GNAT family N-acetyltransferase	-
HPY12_RS15770	379511..380383	+	873	Protein_373	DUF3800 domain-containing protein	-
HPY12_RS15775	380533..381132	+	600	WP_001891245.1	glutathione S-transferase N-terminal domain-containing protein	-
HPY12_RS15780	381189..381293	+	105	WP_099607150.1	acetyltransferase	-
HPY12_RS15785	381324..381593	+	270	WP_001114075.1	DUF4160 domain-containing protein	-
HPY12_RS15790	381587..382108	+	522	WP_000921691.1	DUF2442 domain-containing protein	-
HPY12_RS15795	382407..382532	+	126	WP_001905700.1	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	306166..386842	80676
	-	inside	Integron	-	-	307330..385669	78339
	-	inside	IScluster/Tn	-	-	374870..379054	4184

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (57-133)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 20171.46 Da
• Isoelectric Point: 9.1432

>T157867 WP_000118351.1 NZ_CP053795:377070-377603 [Vibrio cholerae]
VSWGKEFVELSKSKHDRDSFDCGEQELNTFIKTQAAKHMQAGISRTMVLPSAHPLLNQKFAICAFYSVAPSSISRETLPA
QLAKKLPRYPIPVFLLAQLAVHKEFHGSGLGKVSLIRALKYLWEVNHHMRAYAIVVDCLTDSAQAFYTKFGFEVLCEHNE
RIRMFLPMKTVEQLFNQ

Nucleotide

• Length: 534 bp

>T157867 NZ_CP069500:c925807-925553 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCAGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15714.41 Da
• Isoelectric Point: 7.6659

>AT157867 WP_001882332.1 NZ_CP053795:376648-377073 [Vibrio cholerae]
MLCLSLVVMRCQPLRRALYSPSPRKLSVRIECALSLAYNRSTDGMYPYAGGVMATARLDIRLDEEIKAKAEKASALLGLK
SLTEYVVRLMDEDATHVIEEHESIMVKDSVFDEFMAACNKVKAPNQALLEAVKFTEKSGIK

Nucleotide

• Length: 426 bp

>AT157867 NZ_CP069500:c926055-925804 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCCCTGGAGGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCTGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Toxin

• AlphaFold DB: Q9KM94