Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 370179..370945 Replicon chromosome
Accession NZ_CP053795
Organism Vibrio cholerae strain W10G

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag HPY12_RS15675 Protein ID WP_000982260.1
Coordinates 370179..370676 (-) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9K2J6
Locus tag HPY12_RS15680 Protein ID WP_000246253.1
Coordinates 370673..370945 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPY12_RS15635 365632..366171 + 540 WP_057569092.1 phosphoglycerate mutase family protein -
HPY12_RS15640 366261..366842 + 582 WP_043990376.1 nucleotidyltransferase family protein -
HPY12_RS15645 366980..367579 + 600 WP_043990194.1 glutathione S-transferase N-terminal domain-containing protein -
HPY12_RS15650 367764..368177 + 414 WP_000049417.1 VOC family protein -
HPY12_RS15655 368390..368641 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
HPY12_RS15660 368631..368918 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin -
HPY12_RS15665 369046..369502 + 457 Protein_352 GNAT family N-acetyltransferase -
HPY12_RS15670 369824..369976 + 153 WP_001884520.1 DUF645 family protein -
HPY12_RS15675 370179..370676 - 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
HPY12_RS15680 370673..370945 - 273 WP_000246253.1 DUF1778 domain-containing protein Antitoxin
HPY12_RS15685 371063..372022 - 960 WP_001898459.1 IS30 family transposase -
HPY12_RS15690 372311..372568 + 258 WP_000861987.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
HPY12_RS15695 372556..372858 + 303 WP_000229325.1 type II toxin-antitoxin system RelE/ParE family toxin -
HPY12_RS15700 373146..373370 + 225 WP_161571222.1 DUF3709 domain-containing protein -
HPY12_RS15705 373811..373897 + 87 WP_134820423.1 DUF645 family protein -
HPY12_RS15710 373897..373989 + 93 Protein_361 DUF645 family protein -
HPY12_RS15715 374140..374808 + 669 WP_002033340.1 hypothetical protein -
HPY12_RS15720 374870..375307 - 438 WP_000503164.1 IS200/IS605-like element IS1004 family transposase -
HPY12_RS15725 375421..375543 + 123 WP_154591787.1 DUF3265 domain-containing protein -
HPY12_RS15730 375649..375684 + 36 WP_172884631.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 306166..386842 80676
- inside Integron - - 307330..385669 78339
- inside IScluster/Tn - - 374870..379054 4184


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T157865 WP_000982260.1 NZ_CP053795:c370676-370179 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T157865 NZ_CP069500:c617243-617141 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9683.22 Da        Isoelectric Point: 6.2988

>AT157865 WP_000246253.1 NZ_CP053795:c370945-370673 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT157865 NZ_CP069500:617103-617371 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
PDB 1Y9B
AlphaFold DB A0A0F2IC79

References