Detailed information of TA system TA157860

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 350291..350829
• Replicon: chromosome
• Accession: NZ_CP053795
• Organism: Vibrio cholerae strain W10G

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A3Q0L5T8
• Locus tag: HPY12_RS15495
• Protein ID: WP_001247271.1
• Coordinates: 350291..350590 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A0F2HR74
• Locus tag: HPY12_RS15500
• Protein ID: WP_011080384.1
• Coordinates: 350587..350829 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY12_RS15460	345606..345785	+	180	WP_148524399.1	DUF645 family protein	-
HPY12_RS15465	345986..346549	+	564	WP_032469482.1	hypothetical protein	-
HPY12_RS15470	346783..347742	-	960	WP_001898459.1	IS30 family transposase	-
HPY12_RS15475	347641..348366	+	726	WP_175234247.1	hypothetical protein	-
HPY12_RS15480	348503..348937	+	435	WP_000256034.1	GNAT family N-acetyltransferase	-
HPY12_RS15485	349102..349638	+	537	WP_057571969.1	GNAT family N-acetyltransferase	-
HPY12_RS15490	349936..350115	+	180	WP_057569084.1	DUF645 family protein	-
HPY12_RS15495	350291..350590	-	300	WP_001247271.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HPY12_RS15500	350587..350829	-	243	WP_011080384.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
HPY12_RS15505	351528..352298	+	771	WP_057569086.1	alpha/beta hydrolase	-
HPY12_RS15510	352590..352772	+	183	WP_019281482.1	DUF645 family protein	-
HPY12_RS15515	352887..352970	+	84	WP_080099571.1	acetyltransferase	-
HPY12_RS15520	352983..353342	+	360	WP_175234259.1	VOC family protein	-
HPY12_RS15525	353639..353818	+	180	WP_080389342.1	DUF645 family protein	-
HPY12_RS15530	354019..354507	+	489	WP_057569088.1	hypothetical protein	-
HPY12_RS15535	355097..355612	+	516	Protein_326	ClbS/DfsB family four-helix bundle protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	306166..386842	80676
	-	inside	Integron	-	-	307330..385669	78339

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-92)

Antitoxin

• Antitoxin ParD (1-78)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11703.39 Da
• Isoelectric Point: 9.9572

>T157860 WP_001247271.1 NZ_CP053795:c350590-350291 [Vibrio cholerae]
MRPFQLTNKAKSDLRDIALFTSRRWGREQRNIYLKQFDDSFWLLAENPDIGKACDEIRDGYRKFPQGSHVIFYRQIGSQN
IEIIRILHKSMDVNPIFGA

Nucleotide

• Length: 300 bp

>T157860 NZ_CP069499:c109367-109092 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTAATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAATGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8953.88 Da
• Isoelectric Point: 4.2213

>AT157860 WP_011080384.1 NZ_CP053795:c350829-350587 [Vibrio cholerae]
MAKNTSITLGDHFDGFIANQIQSGRYGSASEVIRSALRLLETQETKMNTLRQLLVEGEESGVADYDLDSFINELDSEERK

Nucleotide

• Length: 243 bp

>AT157860 NZ_CP069499:c109651-109367 [Escherichia coli]
ATACAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCACTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGATTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A3Q0L5T8

Antitoxin

• AlphaFold DB: A0A0F2HR74