Detailed information of TA system TA157823

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 815007..815805
• Replicon: chromosome
• Accession: NZ_CP053787
• Organism: Escherichia coli isolate J31

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A166U9J9
• Locus tag: HQN81_RS03885
• Protein ID: WP_000854695.1
• Coordinates: 815428..815805 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A2U2UUE3
• Locus tag: HQN81_RS03880
• Protein ID: WP_016243360.1
• Coordinates: 815007..815381 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HQN81_RS03860	812683..813501	+	819	WP_001241366.1	DUF945 domain-containing protein	-
HQN81_RS03865	813583..814062	+	480	WP_000860084.1	antirestriction protein	-
HQN81_RS03870	814078..814554	+	477	WP_001186773.1	RadC family protein	-
HQN81_RS03875	814623..814844	+	222	WP_000692307.1	DUF987 domain-containing protein	-
HQN81_RS03880	815007..815381	+	375	WP_016243360.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HQN81_RS03885	815428..815805	+	378	WP_000854695.1	TA system toxin CbtA family protein	Toxin
HQN81_RS03890	815802..816290	+	489	WP_000761710.1	hypothetical protein	-
HQN81_RS03895	816302..816499	+	198	WP_000839228.1	DUF957 domain-containing protein	-
HQN81_RS03900	816584..817432	+	849	WP_001280536.1	DUF4942 domain-containing protein	-
HQN81_RS03905	817525..818844	-	1320	WP_000144689.1	site-specific integrase	-
HQN81_RS03910	819181..819591	-	411	WP_001111348.1	hypothetical protein	-
HQN81_RS03915	819570..820526	-	957	WP_172614063.1	molybdenum cofactor insertion chaperone PaoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	814078..825404	11326

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14062.03 Da
• Isoelectric Point: 7.7531

>T157823 WP_000854695.1 NZ_CP053787:815428-815805 [Escherichia coli]
MKTLPDTHVREASCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPGAKQ

Nucleotide

• Length: 378 bp

>T157823 NZ_CP069488:c212672-212208 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13810.66 Da
• Isoelectric Point: 7.0266

>AT157823 WP_016243360.1 NZ_CP053787:815007-815381 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT157823 NZ_CP069488:c213007-212672 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A166U9J9

Antitoxin

• AlphaFold DB: A0A2U2UUE3