Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 473622..474198 Replicon chromosome
Accession NZ_CP053787
Organism Escherichia coli isolate J31

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag HQN81_RS02260 Protein ID WP_001297643.1
Coordinates 473622..473909 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag HQN81_RS02265 Protein ID WP_000063156.1
Coordinates 473896..474198 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HQN81_RS02230 468781..469038 - 258 WP_000657676.1 DUF3343 domain-containing protein -
HQN81_RS02235 469035..469802 - 768 WP_000222495.1 hypothetical protein -
HQN81_RS02240 469812..470963 - 1152 WP_001298064.1 2-hydroxyacyl-CoA dehydratase subunit D -
HQN81_RS02245 471079..472359 - 1281 WP_119188294.1 DUF445 domain-containing protein -
HQN81_RS02250 472400..472669 - 270 Protein_432 MFS transporter -
HQN81_RS02255 472726..473423 + 698 Protein_433 IS1 family transposase -
HQN81_RS02260 473622..473909 + 288 WP_001297643.1 BrnT family toxin Toxin
HQN81_RS02265 473896..474198 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
HQN81_RS02270 474232..475188 - 957 WP_001297640.1 GTPase -
HQN81_RS02275 475199..475402 - 204 WP_000467859.1 YbdD/YjiX family protein -
HQN81_RS02280 475452..477602 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T157821 WP_001297643.1 NZ_CP053787:473622-473909 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T157821 NZ_CP069486:1669825-1670196 [Mammaliicoccus vitulinus]
ATGAGAAGAGGAGATGTATATCTCGCAGATCTTTCACCAGTCACTGGTTCTGAACAAGGGGGGACAAGACCAGTCGTAAT
TATCCAAAATGATACGGGTAACAGATATAGTCCGACTGTTATTGTAGCAGCAATTACTGGTAAGATAAATAAAGCTAAAA
TACCTACACACGTAGAAATTGAAGCAGCGAAATATAAACTAGATAGAGATTCAGTTATTTTGCTAGAACAAATTAGAACC
ATTGATAAGAAACGTTTAAAAGAAAAATTAACATATTTATCTGATGCAAAGATGAAAGAGGTTGATACAGCTATAGCAAT
CAGCCTCAACCTAACTTTGCAACAAAAATTTGATGCATTAGGTAATACATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT157821 WP_000063156.1 NZ_CP053787:473896-474198 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT157821 NZ_CP069486:1669657-1669824 [Mammaliicoccus vitulinus]
ATGACATCTTTAACACAAAATCGTACTCAAAGTATAGAACAAACACTGAAAGAAGGATATCGTTCTATGGCTGATTTAAA
TCTCTCCCTTGCATCTGAAGCATACTATAGTGAATGTGAAGCTTGTGATTCTAATGAATCGCACTTAGCATCGCAACAAG
GTGAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References