Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3444069..3444687 Replicon chromosome
Accession NZ_CP053785
Organism Escherichia coli isolate 2-101

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HQN80_RS16600 Protein ID WP_001291435.1
Coordinates 3444469..3444687 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HQN80_RS16595 Protein ID WP_000344800.1
Coordinates 3444069..3444443 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HQN80_RS16585 3439158..3440351 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HQN80_RS16590 3440374..3443523 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
HQN80_RS16595 3444069..3444443 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HQN80_RS16600 3444469..3444687 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
HQN80_RS16605 3444859..3445410 + 552 WP_000102568.1 maltose O-acetyltransferase -
HQN80_RS16610 3445526..3445996 + 471 WP_000136192.1 YlaC family protein -
HQN80_RS16615 3446160..3447710 + 1551 WP_001372022.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HQN80_RS16620 3447752..3448105 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HQN80_RS16630 3448484..3448795 + 312 WP_000409911.1 MGMT family protein -
HQN80_RS16635 3448826..3449398 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T157812 WP_001291435.1 NZ_CP053785:3444469-3444687 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T157812 NZ_CP069480:4602429-4602680 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT157812 WP_000344800.1 NZ_CP053785:3444069-3444443 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT157812 NZ_CP069480:4602677-4602931 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References