Detailed information of TA system TA157802

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1846766..1847601
• Replicon: chromosome
• Accession: NZ_CP053785
• Organism: Escherichia coli isolate 2-101

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1U9SHU4
• Locus tag: HQN80_RS08775
• Protein ID: WP_000854752.1
• Coordinates: 1846766..1847143 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E3PJ72
• Locus tag: HQN80_RS08780
• Protein ID: WP_001278232.1
• Coordinates: 1847233..1847601 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HQN80_RS08735	1842286..1842759	+	474	WP_001105384.1	DNA gyrase inhibitor SbmC	-
HQN80_RS08740	1842957..1844015	+	1059	WP_001200892.1	FUSC family protein	-
HQN80_RS08745	1844187..1844516	+	330	WP_000450409.1	DUF496 family protein	-
HQN80_RS08750	1844617..1844799	-	183	WP_024167529.1	ethanolamine utilization protein	-
HQN80_RS08755	1844812..1844982	-	171	Protein_1712	propanediol utilization protein	-
HQN80_RS08760	1845253..1846281	-	1029	Protein_1713	IS110 family transposase	-
HQN80_RS08765	1846440..1846553	-	114	WP_001161660.1	DUF957 domain-containing protein	-
HQN80_RS08770	1846566..1846769	-	204	WP_000761705.1	hypothetical protein	-
HQN80_RS08775	1846766..1847143	-	378	WP_000854752.1	TA system toxin CbtA family protein	Toxin
HQN80_RS08780	1847233..1847601	-	369	WP_001278232.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
HQN80_RS08785	1847764..1847985	-	222	WP_000692329.1	DUF987 domain-containing protein	-
HQN80_RS08790	1848048..1848524	-	477	WP_001347688.1	RadC family protein	-
HQN80_RS08795	1848540..1849025	-	486	WP_000849582.1	antirestriction protein	-
HQN80_RS08800	1849080..1849175	-	96	Protein_1721	DUF945 domain-containing protein	-
HQN80_RS08805	1849277..1850032	-	756	WP_001282653.1	IS21-like element ISSso4 family helper ATPase IstB	-
HQN80_RS08810	1850049..1851584	-	1536	WP_023181050.1	IS21 family transposase	-
HQN80_RS08815	1851818..1852543	-	726	Protein_1724	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14076.08 Da
• Isoelectric Point: 8.2905

>T157802 WP_000854752.1 NZ_CP053785:c1847143-1846766 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T157802 NZ_CP069480:1695268-1695609 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACTGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTCGCCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT157802 WP_001278232.1 NZ_CP053785:c1847601-1847233 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT157802 NZ_CP069480:c1695273-1695197 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A1U9SHU4

Antitoxin

• AlphaFold DB: E3PJ72