Detailed information of TA system TA157800

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1324738..1325363
• Replicon: chromosome
• Accession: NZ_CP053785
• Organism: Escherichia coli isolate 2-101

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HQN80_RS06380
• Protein ID: WP_000911330.1
• Coordinates: 1324965..1325363 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: HQN80_RS06375
• Protein ID: WP_000450524.1
• Coordinates: 1324738..1324965 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HQN80_RS06350	1320541..1321011	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
HQN80_RS06355	1321011..1321583	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
HQN80_RS06360	1321729..1322607	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
HQN80_RS06365	1322624..1323658	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
HQN80_RS06370	1323871..1324584	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
HQN80_RS06375	1324738..1324965	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
HQN80_RS06380	1324965..1325363	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HQN80_RS06385	1325510..1326373	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
HQN80_RS06390	1326388..1328403	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
HQN80_RS06395	1328477..1329175	+	699	WP_000679823.1	esterase	-
HQN80_RS06400	1329285..1329485	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T157800 WP_000911330.1 NZ_CP053785:1324965-1325363 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T157800 NZ_CP069480:c1533527-1533213 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT157800 WP_000450524.1 NZ_CP053785:1324738-1324965 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT157800 NZ_CP069480:c1533763-1533530 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A829CM33