Detailed information of TA system TA157793

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 81944..82980
• Replicon: plasmid pEI-2234-3
• Accession: NZ_CP053782
• Organism: Edwardsiella ictaluri strain 2234

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: F8538_RS17710
• Protein ID: WP_160188649.1
• Coordinates: 81944..82519 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: F8538_RS17715
• Protein ID: WP_152112795.1
• Coordinates: 82519..82980 (-)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F8538_RS17700	77061..77786	+	726	WP_116547374.1	hypothetical protein	-
F8538_RS17705	77966..81724	+	3759	WP_198089506.1	hypothetical protein	-
F8538_RS17710	81944..82519	-	576	WP_160188649.1	PIN domain-containing protein	Toxin
F8538_RS17715	82519..82980	-	462	WP_152112795.1	helix-turn-helix domain-containing protein	Antitoxin
F8538_RS17720	83468..84466	+	999	WP_198089507.1	tyrosine-type recombinase/integrase	-
F8538_RS17725	84526..85017	+	492	WP_198089515.1	hypothetical protein	-
F8538_RS17730	85168..86187	-	1020	Protein_96	transposase	-
F8538_RS17735	86479..87135	+	657	WP_198089508.1	QnrS family quinolone resistance pentapeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(A) / dfrA1 / qacE / sul1 / floR / sul2 / qnrS5	-	1..109462	109462
	-	flank	IS/Tn	qnrS5	-	85297..87135	1838

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-115)

Antitoxin

• SinI-like, DNA-binding domain (84-129)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21504.97 Da
• Isoelectric Point: 4.6863

>T157793 WP_160188649.1 NZ_CP053782:c82519-81944 [Edwardsiella ictaluri]
MKHTPYPVVLDACVLYPSFLRDLLIRLGLTGLYQPKWSAIIEDEWQRNLLADRTDLTLEQVQRTATLMNKAVPDAMITGF
EPLIDSIVLPDVDDRHVVAAAVRSNAEIIVTLNLKDFPTPALDTFGIEALHPDDFIMDLFDLNSALVLSAVSMQRSNLRK
PPMSVDDYLAALLRQGMAQTVKELNKYRQLI

Nucleotide

• Length: 576 bp

>T157793 NZ_CP069473:1551402-1551581 [Staphylococcus epidermidis]
ATGGCGAACAAAAAAGATTCCAAATTAAATTATCATGAAGAAGAAAATGCTATGGTTACCGACTTAGATGATTTAAAAGA
ATTAGGAAAAGAAATGGAACAGATTTCACAGGAAAATGATGAAGAAAAATTAAATCAATCTCATGATAACGAAGTTCGTT
CAGATTTAAAAAAACAGTAA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 16933.35 Da
• Isoelectric Point: 6.2243

>AT157793 WP_152112795.1 NZ_CP053782:c82980-82519 [Edwardsiella ictaluri]
MTNSILDNLSLPAKGEIEAAVRGQRDLAAYLSTKVETQKIAIQDADNVTHHIELPTSSLKLLEAILGELALGNAVQVVPV
HAELTTQEAANILNVSRPHMVKLLEEGQLPFHKTGRHRRVLFANLMQYKDKRDKESNSAMQELADLSQDLGLY

Nucleotide

• Length: 462 bp

>AT157793 NZ_CP069473:1550779-1551378 [Staphylococcus epidermidis]
ATGGCAATGAATTTTAAAGTATTTGATAATAGTGAAAAAGTAGCAGAATATACTGCAGATATTCTACGTAAGCAATTTAA
TAATAATCCAACTACTATTGCAGGATTTCATTTGAGCAAAGAACATGCACCAGTGTTTGATGAACTTAAGAAAAATGTAG
AAAATCATACAGTTGATTTCAGTCAAATAAATATTTTAGATTATGATGATAATCATTCTTATTATGAGGCTTTGGGTGTG
CCAACAGGCCAAATTTATTCCATTTCTTACGAAAATGATGCAATTGACTTTATCTCAGATAAGATTAAGACTAAAGAAAA
TAAAGGCAAACTGACAATGCAAGTCCTTACGATTGATGAAAATGGTAAACTTGACATTAGCGTTCGTCAAGGTTTGATGG
AAGCTAGAGAAATTTTCTTAGTTATTACTGGTGCTAATAAACGCGATATGGTAGAAAAACTTTACCGTGAAAATGGTAAG
ACTAGTTTTGAACCATCGGATTTAAAAGCACACCGTATGGTCAATGTTATCTTAGATAAAGAAGCGGCTGCAGGCTTACC
TGAAGACGTAAAAGAGTACTTCACTGCACGTTTTGCATAA