Detailed information of TA system TA157788

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 119102..119772
• Replicon: plasmid pvirulence_VBP3636
• Accession: NZ_CP053772
• Organism: Klebsiella pneumoniae strain BP3636

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: HBJ33_RS26450
• Protein ID: WP_004213072.1
• Coordinates: 119329..119772 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: HBJ33_RS26445
• Protein ID: WP_004213073.1
• Coordinates: 119102..119332 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBJ33_RS26420	115325..116224	-	900	WP_004225022.1	nucleotide-binding protein	-
HBJ33_RS26425	116214..116504	-	291	WP_004213078.1	hypothetical protein	-
HBJ33_RS26430	116856..117062	+	207	WP_004213077.1	hypothetical protein	-
HBJ33_RS26435	117052..117345	-	294	WP_004213076.1	hypothetical protein	-
HBJ33_RS26440	117361..118494	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
HBJ33_RS26445	119102..119332	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HBJ33_RS26450	119329..119772	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
HBJ33_RS26455	119786..120202	-	417	Protein_144	DUF3644 domain-containing protein	-
HBJ33_RS26460	120206..120499	-	294	Protein_145	transposase	-
HBJ33_RS26465	120916..121550	+	635	Protein_146	response regulator	-
HBJ33_RS26470	122066..122472	-	407	Protein_147	GAF domain-containing protein	-
HBJ33_RS26475	122563..123483	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
HBJ33_RS26480	123532..124023	-	492	WP_011251327.1	DM13 domain-containing protein	-
HBJ33_RS26485	124086..124361	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-15 / blaTEM-1A / tet(D) / dfrA14 / blaOXA-1 / aac(6')-Ib-cr / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12	rmpA / iutA / iucD / iucC / iucB / iucA	1..317680	317680

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T157788 WP_004213072.1 NZ_CP053772:119329-119772 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T157788 NZ_CP069470:909997-910149 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT157788 WP_004213073.1 NZ_CP053772:119102-119332 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT157788 NZ_CP069470:909373-909972 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620