Detailed information of TA system TA157787

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 73007..73758
• Replicon: plasmid pvirulence_VBP3636
• Accession: NZ_CP053772
• Organism: Klebsiella pneumoniae strain BP3636

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: HBJ33_RS26205
• Protein ID: WP_048333033.1
• Coordinates: 73276..73758 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: HBJ33_RS26200
• Protein ID: WP_004902250.1
• Coordinates: 73007..73285 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBJ33_RS26165	68646..69071	-	426	WP_000522996.1	organomercurial transporter MerC	-
HBJ33_RS26170	69099..69374	-	276	WP_043907009.1	mercury resistance system periplasmic binding protein MerP	-
HBJ33_RS26175	69390..69755	-	366	WP_004200999.1	mercuric ion transporter MerT	-
HBJ33_RS26180	69827..70282	+	456	WP_001166628.1	Hg(II)-responsive transcriptional regulator	-
HBJ33_RS26185	70935..71393	+	459	WP_014386535.1	hypothetical protein	-
HBJ33_RS26190	72224..72565	+	342	WP_032437747.1	hypothetical protein	-
HBJ33_RS26195	72673..72885	+	213	WP_016946198.1	hypothetical protein	-
HBJ33_RS26200	73007..73285	+	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
HBJ33_RS26205	73276..73758	+	483	WP_048333033.1	GNAT family N-acetyltransferase	Toxin
HBJ33_RS26210	74793..75332	+	540	WP_004902239.1	hypothetical protein	-
HBJ33_RS26215	75437..75829	+	393	WP_032442757.1	hypothetical protein	-
HBJ33_RS26220	75930..76685	+	756	WP_004902235.1	DUF2971 domain-containing protein	-
HBJ33_RS26225	76712..77359	+	648	WP_014386537.1	EcsC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-15 / blaTEM-1A / tet(D) / dfrA14 / blaOXA-1 / aac(6')-Ib-cr / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12	rmpA / iutA / iucD / iucC / iucB / iucA	1..317680	317680

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17708.53 Da
• Isoelectric Point: 8.9130

>T157787 WP_048333033.1 NZ_CP053772:73276-73758 [Klebsiella pneumoniae]
MGMRAPESLMSEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T157787 NZ_CP069470:806709-806885 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT157787 WP_004902250.1 NZ_CP053772:73007-73285 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT157787 NZ_CP069470:c807047-806873 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Structures

Antitoxin

• AlphaFold DB: A0A071LPN3