Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 3495177..3495763 Replicon chromosome
Accession NZ_CP053771
Organism Klebsiella pneumoniae strain BP3636

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag HBJ33_RS16585 Protein ID WP_002920800.1
Coordinates 3495395..3495763 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag HBJ33_RS16580 Protein ID WP_004174006.1
Coordinates 3495177..3495398 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HBJ33_RS16560 3491334..3492260 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
HBJ33_RS16565 3492257..3493534 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
HBJ33_RS16570 3493531..3494298 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
HBJ33_RS16575 3494300..3495013 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
HBJ33_RS16580 3495177..3495398 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HBJ33_RS16585 3495395..3495763 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
HBJ33_RS16590 3496036..3497352 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
HBJ33_RS16595 3497459..3498346 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
HBJ33_RS16600 3498343..3499188 + 846 WP_004181473.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
HBJ33_RS16605 3499190..3500260 + 1071 WP_043906884.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3490163..3500997 10834


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T157777 WP_002920800.1 NZ_CP053771:3495395-3495763 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T157777 NZ_CP069460:34038-34187 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT157777 WP_004174006.1 NZ_CP053771:3495177-3495398 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT157777 NZ_CP069460:c33990-33934 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References