Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3056777..3057402 Replicon chromosome
Accession NZ_CP053771
Organism Klebsiella pneumoniae strain BP3636

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HBJ33_RS14555 Protein ID WP_002882817.1
Coordinates 3056777..3057160 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HBJ33_RS14560 Protein ID WP_004150355.1
Coordinates 3057160..3057402 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HBJ33_RS14540 3054143..3055045 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HBJ33_RS14545 3055042..3055677 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HBJ33_RS14550 3055674..3056603 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HBJ33_RS14555 3056777..3057160 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HBJ33_RS14560 3057160..3057402 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HBJ33_RS14565 3057607..3058524 + 918 WP_004181612.1 alpha/beta hydrolase -
HBJ33_RS14570 3058538..3059479 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HBJ33_RS14575 3059524..3059961 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HBJ33_RS14580 3059958..3060818 - 861 WP_004146232.1 virulence factor BrkB family protein -
HBJ33_RS14585 3060812..3061411 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T157776 WP_002882817.1 NZ_CP053771:c3057160-3056777 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T157776 NZ_CP069460:10764-11069 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT157776 WP_004150355.1 NZ_CP053771:c3057402-3057160 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT157776 NZ_CP069460:10544-10762 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References