Detailed information of TA system TA157762

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 123292..123962
• Replicon: plasmid pvirulence_VBA10835
• Accession: NZ_CP053766
• Organism: Klebsiella pneumoniae strain BA10835

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: HBJ44_RS26410
• Protein ID: WP_004213072.1
• Coordinates: 123292..123735 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: HBJ44_RS26415
• Protein ID: WP_004213073.1
• Coordinates: 123732..123962 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBJ44_RS26375	118703..118978	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
HBJ44_RS26380	119041..119532	+	492	WP_011251327.1	DM13 domain-containing protein	-
HBJ44_RS26385	119581..120501	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
HBJ44_RS26390	120592..120998	+	407	Protein_131	GAF domain-containing protein	-
HBJ44_RS26395	121514..122148	-	635	Protein_132	response regulator	-
HBJ44_RS26400	122565..122858	+	294	Protein_133	transposase	-
HBJ44_RS26405	122862..123278	+	417	Protein_134	DUF3644 domain-containing protein	-
HBJ44_RS26410	123292..123735	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
HBJ44_RS26415	123732..123962	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HBJ44_RS26420	124570..125703	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
HBJ44_RS26425	125719..126012	+	294	WP_004213076.1	hypothetical protein	-
HBJ44_RS26430	126002..126208	-	207	WP_004213077.1	hypothetical protein	-
HBJ44_RS26435	126560..126850	+	291	WP_004213078.1	hypothetical protein	-
HBJ44_RS26440	126840..127739	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-15 / blaTEM-1B / tet(D) / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12	iucA / iucB / iucC / iucD / iutA / rmpA	1..307718	307718

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T157762 WP_004213072.1 NZ_CP053766:c123735-123292 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T157762 NZ_CP069459:c929049-928762 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT157762 WP_004213073.1 NZ_CP053766:c123962-123732 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT157762 NZ_CP069459:c929288-929049 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620