Detailed information of TA system TA157743

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1248100..1249017
• Replicon: chromosome
• Accession: NZ_CP053764
• Organism: Bacillus velezensis strain B268

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: HPQ66_RS06345
• Protein ID: WP_057079950.1
• Coordinates: 1248271..1249017 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: HPQ66_RS06340
• Protein ID: WP_003154807.1
• Coordinates: 1248100..1248270 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPQ66_RS06305	1243312..1244901	+	1590	WP_077722100.1	hypothetical protein	-
HPQ66_RS06310	1244914..1245339	+	426	WP_077722101.1	hypothetical protein	-
HPQ66_RS06315	1245344..1245541	+	198	WP_168237133.1	XkdX family protein	-
HPQ66_RS06320	1245598..1246359	+	762	WP_146276587.1	phage portal protein	-
HPQ66_RS06325	1246411..1246674	+	264	WP_146276589.1	hemolysin XhlA family protein	-
HPQ66_RS06330	1246688..1246951	+	264	WP_003154813.1	phage holin	-
HPQ66_RS06335	1246965..1247843	+	879	WP_058905962.1	N-acetylmuramoyl-L-alanine amidase	-
HPQ66_RS06340	1248100..1248270	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
HPQ66_RS06345	1248271..1249017	-	747	WP_057079950.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
HPQ66_RS06350	1249122..1250120	-	999	WP_003154805.1	inorganic phosphate transporter	-
HPQ66_RS06355	1250133..1250750	-	618	WP_003154804.1	DUF47 domain-containing protein	-
HPQ66_RS06360	1251036..1252352	-	1317	WP_172614618.1	amino acid permease	-
HPQ66_RS06365	1252675..1253625	+	951	WP_077722102.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29033.52 Da
• Isoelectric Point: 4.7755

>T157743 WP_057079950.1 NZ_CP053764:c1249017-1248271 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T157743 NZ_CP069453:c2819933-2819715 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT157743 WP_003154807.1 NZ_CP053764:c1248270-1248100 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT157743 NZ_CP069453:c2820333-2819959 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: I2HQ14