Detailed information of TA system TA157742

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 516459..517096
• Replicon: chromosome
• Accession: NZ_CP053764
• Organism: Bacillus velezensis strain B268

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: HPQ66_RS02485
• Protein ID: WP_003156187.1
• Coordinates: 516746..517096 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: HPQ66_RS02480
• Protein ID: WP_003156188.1
• Coordinates: 516459..516740 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPQ66_RS02460	512824..513423	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
HPQ66_RS02465	513516..513881	+	366	WP_071392104.1	holo-ACP synthase	-
HPQ66_RS02470	514046..515053	+	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
HPQ66_RS02475	515170..516339	+	1170	WP_077721837.1	alanine racemase	-
HPQ66_RS02480	516459..516740	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
HPQ66_RS02485	516746..517096	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
HPQ66_RS02490	517214..518035	+	822	WP_003156182.1	STAS domain-containing protein	-
HPQ66_RS02495	518040..518405	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
HPQ66_RS02500	518408..518809	+	402	WP_003156178.1	anti-sigma regulatory factor	-
HPQ66_RS02505	518821..519828	+	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
HPQ66_RS02510	519892..520221	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
HPQ66_RS02515	520218..520700	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
HPQ66_RS02520	520666..521454	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
HPQ66_RS02525	521454..522056	+	603	WP_146276154.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T157742 WP_003156187.1 NZ_CP053764:516746-517096 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T157742 NZ_CP069453:2621486-2621884 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT157742 WP_003156188.1 NZ_CP053764:516459-516740 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT157742 NZ_CP069453:2621190-2621483 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5