Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 34294..34919 Replicon plasmid pMHMC-001
Accession NZ_CP053752
Organism Shigella sonnei strain 506

Toxin (Protein)


Gene name vapC Uniprot ID Q6XVU6
Locus tag COO90_RS24500 Protein ID WP_000911318.1
Coordinates 34521..34919 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q31SG2
Locus tag COO90_RS24495 Protein ID WP_000450530.1
Coordinates 34294..34521 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COO90_RS24490 (COO90_24310) 29942..34212 - 4271 Protein_39 conjugative transfer relaxase/helicase TraI -
COO90_RS24495 (COO90_24320) 34294..34521 + 228 WP_000450530.1 toxin-antitoxin system antitoxin VapB Antitoxin
COO90_RS24500 (COO90_24325) 34521..34919 + 399 WP_000911318.1 type II toxin-antitoxin system VapC family toxin Toxin
COO90_RS24505 (COO90_24330) 34928..37084 - 2157 WP_052945385.1 type IV conjugative transfer system coupling protein TraD -
COO90_RS24510 (COO90_24335) 37110..37241 - 132 Protein_43 IS1 family transposase -
COO90_RS24520 (COO90_24345) 38506..38844 - 339 Protein_45 antirestriction protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - tviC / ipaH1.4 / ospG / ipaH9.8 / ospI / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaA / ipaJ / ospC3 / ospC2 / ipaH4.5 / ipaH7.8 / ospD3/senA / ospC1 / ospE2 / ipgB2 / ospD1 / ospF / ospD2 / ospC4 / ospB / icsP/sopA / nleE 1..212834 212834


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14845.09 Da        Isoelectric Point: 8.5264

>T157730 WP_000911318.1 NZ_CP053752:34521-34919 [Shigella sonnei]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRTEDWS

Download         Length: 399 bp

>T157730 NZ_CP069451:4536757-4537221 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAGCCATTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8535.59 Da        Isoelectric Point: 4.7513

>AT157730 WP_000450530.1 NZ_CP053752:34294-34521 [Shigella sonnei]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHSVSADFMDNREQPGMQERESF

Download         Length: 228 bp

>AT157730 NZ_CP069451:4536422-4536757 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6SD6

Antitoxin

Source ID Structure
AlphaFold DB Q31SG2

References