Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 20439..20974 Replicon plasmid pMHMC-001
Accession NZ_CP053752
Organism Shigella sonnei strain 506

Toxin (Protein)


Gene name relE Uniprot ID Q3YTD6
Locus tag COO90_RS24410 Protein ID WP_000222766.1
Coordinates 20439..20726 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0I0JNN8
Locus tag COO90_RS24415 Protein ID WP_001132903.1
Coordinates 20723..20974 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COO90_RS24375 (COO90_24195) 15614..15711 + 98 Protein_16 IS1 family transposase -
COO90_RS24380 (COO90_24200) 15868..16088 + 221 Protein_17 hypothetical protein -
COO90_RS24385 (COO90_24205) 16478..18205 + 1728 WP_000597732.1 T3SS effector E3 ubiquitin-protein ligase IpaH1.4 -
COO90_RS24390 (COO90_24210) 18235..18390 - 156 Protein_19 IS256 family transposase -
COO90_RS24395 (COO90_24215) 18529..18687 - 159 Protein_20 transposase -
COO90_RS24400 (COO90_24220) 18809..18997 + 189 WP_000957853.1 hypothetical protein -
COO90_RS24405 (COO90_24225) 19007..20206 + 1200 Protein_22 IS91 family transposase -
COO90_RS24410 (COO90_24230) 20439..20726 - 288 WP_000222766.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
COO90_RS24415 (COO90_24235) 20723..20974 - 252 WP_001132903.1 plasmid stabilization protein Antitoxin
COO90_RS24420 (COO90_24240) 21047..21238 - 192 WP_000829591.1 hypothetical protein -
COO90_RS24425 (COO90_24245) 21938..22795 - 858 WP_000130974.1 incFII family plasmid replication initiator RepA -
COO90_RS24430 (COO90_24250) 22788..22862 - 75 WP_001365705.1 RepA leader peptide Tap -
COO90_RS24435 (COO90_24255) 23086..23346 - 261 WP_000083819.1 replication regulatory protein RepA -
COO90_RS24440 (COO90_24260) 23586..23939 - 354 Protein_29 DUF2726 domain-containing protein -
COO90_RS24445 (COO90_24265) 23996..24693 + 698 WP_252965859.1 IS1-like element IS1A family transposase -
COO90_RS24450 (COO90_24270) 24708..24953 - 246 Protein_31 hypothetical protein -
COO90_RS24455 (COO90_24275) 24992..25201 - 210 WP_001299729.1 hemolysin expression modulator Hha -
COO90_RS24460 (COO90_24280) 25247..25708 - 462 WP_001233865.1 thermonuclease family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - tviC / ipaH1.4 / ospG / ipaH9.8 / ospI / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaA / ipaJ / ospC3 / ospC2 / ipaH4.5 / ipaH7.8 / ospD3/senA / ospC1 / ospE2 / ipgB2 / ospD1 / ospF / ospD2 / ospC4 / ospB / icsP/sopA / nleE 1..212834 212834


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11147.23 Da        Isoelectric Point: 10.5832

>T157729 WP_000222766.1 NZ_CP053752:c20726-20439 [Shigella sonnei]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Download         Length: 288 bp

>T157729 NZ_CP069451:4314949-4315323 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9225.44 Da        Isoelectric Point: 4.0927

>AT157729 WP_001132903.1 NZ_CP053752:c20974-20723 [Shigella sonnei]
MPNIILSETSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT157729 NZ_CP069451:4314491-4314859 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACAGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CDZ2

Antitoxin

Source ID Structure
AlphaFold DB A0A0I0JNN8

References